Herbal Combinations for Treating Eczema

ABSTRACT

A shampoo, conditioner, cream, ointment or other topical treatment includes an active herbal combination of Sheng Di Huang, Da Huang and Jin Yin Hua for treating eczema, and/or another skin or scalp disorder.

PRIORITY AND RELATED APPLICATIONS

This application claims priority to U.S. provisional patent applicationsNo. 62/198,637, filed Jul. 29, 2015 and 62/297,796, filed Feb. 29, 2016.

This application is also a continuation-in-part (CIP) which claimspriority to U.S. patent application Ser. No. 14/754,266, filed Jun. 29,2015.

This application is also a continuation-in-part (CIP) which claimspriority to U.S. patent application Ser. No. 15/131,743, filed Apr. 18,2016.

This application is related to United States patent application serialno. U.S. Ser. No. 13/900,525, filed May 22, 2013; now U.S. Pat. No.9,095,606, which is a CIP of U.S. Ser. No. 13/361,978, filed Jan. 31,2012.

This application is also related to U.S. patent application Ser. Nos.13/900,526, 14/094,535, 13/152,039, and 14/034,423, and to U.S. Pat.Nos. 8,734,859, 8,597,695, 8,541,382, and 9,066,974, and to US publishedpatent application nos. 20140206631 and 20140205685.

All of these priority and related patents and patent applications arehereby incorporated by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates generally to treatments for eczema. Morespecifically it concerns the use of herbal combinations and combinationsof certain herbs, certain herbal extracts and/or certain molecularcomponents of certain herbs, alone or in combination with orsupplemental to one or more other known or novel or experimentaltreatments, formulated as a shampoo, conditioner, cream, ointment orother topical scalp, hair or skin treatment to treat an eczematiccondition.

2. Description of the Related Art

Often, eczema is not detected because it is not visible underneath thehair of the person who is suffering with eczema. Sometimes, head orscalp eczema is confused with dandruff or other head or scalp conditionsthat are sometimes deemed to be treatable with ordinary shampoos andconditioners.

There exist treatments for psoriatic and eczematic scalp conditionswhich are steroid-based. Steroid-based treatments are sometimeseffective, but can also sometimes have serious negative short-term orlong-term side effects. Clobex shampoo (0.05%), by Galderma Labs USA, isan example of a steroid, specifically a corticosteroid, based shampoothat was approved by FDA for treatment of scalp psoriasis. CLOBEX®(clobetasol propionate) Shampoo, 0.05%, contains clobetasol propionate,a synthetic fluorinated corticosteroid, for topical dermatologic use.The corticosteroids constitute a class of primarily synthetic steroidsused topically as anti-inflammatory and antipruritic agents. Clobetasolpropionate is a white to practically white crystalline, odorless powderinsoluble in water. While Clobex shampoo has been shown to beeffectiveness in treating scalp psoriasis in a significant percentage oftest subjects, a large percentage of test subjects were also subjectedto serious negative side effects. It is desired to have an effective andsafe, non-steroidal medicinal formulation for treating eczema, and/orpsoriasis or another skin condition.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates the components of a shampoo in accordance withcertain embodiments.

FIG. 2 illustrates a hair growth cycle and a hair fall control strategyin accordance with certain embodiments.

FIG. 3 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on BodyWeight.

FIG. 4 effects of combinations of Sheng Di Huang, Da Huang and Jin YinHua and of combinations of digoxin and emodin on % Body Weight Change.

FIG. 5 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on AbsoluteEar Thickness.

FIG. 6 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on AbsoluteEar Thickness.

FIG. 7 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on EarThickness Change.

FIG. 8 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on EarThickness Change.

FIG. 9 illustrates effects of combinations of Sheng Di Huang, Da Huangand Jin Yin Hua and of combinations of digoxin and emodin on Ear PunchBiopsy Weight.

FIGS. 10A-10G show photographs of TPA induced inflammation in mice earsthat were represented for all the experimental groups which demonstratedthe gross effect of test items.

FIG. 11 illustrates % Inhibition of Ear Inflammation on topicaltreatment.

FIG. 12 illustrates % Inhibition of Ear Inflammation on oral treatment.

FIGS. 13A-13H illustrate histopathological findings in accordance withcertain embodiments.

FIG. 14 illustrates effects of topical application of Sheng Di Huang, DaHuang and Jin Yin Hua and of combinations of digoxin and emodin onepidermal ear thickness.

FIG. 15 illustrates effects of oral applications of Sheng Di Huang, DaHuang and Jin Yin Hua and of combinations of digoxin and emodin onepidermal ear thickness

FIG. 16 illustrates effects of topical application of Sheng Di Huang, DaHuang and Jin Yin Hua and of combinations of digoxin and emodin on MPOactivity in TPA induced mice ears.

FIG. 17 illustrates effects of oral administration of Sheng Di Huang, DaHuang and Jin Yin Hua and of combinations of digoxin and emodin on MPOactivity in TPA induced mice ears.

FIG. 18 illustrates the % Inhibition of serum Nitric oxide content on ofSheng Di Huang, Da Huang and Jin Yin Hua and of combinations of digoxinand emodin treatment.

FIG. 19 illustrates the effect of proliferation of Digoxin, emodin andtheir combination on MDA 435 cell lines of Melanoma and Breast Cancer.

FIG. 20 is a bar chart that illustrates the effects of certain herbs andcombinations of herbs on live cancer cells, including (C1) Sheng DiHuang—Rhemannia; as well as (C2) the combination of Jin YinHua—Lonicerae, Da Huang—Rhei, Mu Dan Pi—Moutan, and Di Gu Pi—CortexLycii, and (C3) the combination of Xian He Cao—Agrimoniae and Chun GenPI—Ailanthi.

FIGS. 21-23 show plots of growth of cancer cells versus dilution factorfor each of 18 different herbs.

FIG. 24 is a bar chart that illustrates the effects of certain herbs andcombinations of herbs on live cancer cells.

DETAILED DESCRIPTIONS OF THE EMBODIMENTS

Eczema is an inflammatory, chronically relapsing, non-contagious andpruritic disease. Current management strategies include oralmedications, steroid creams and light therapy. We have developed a novelaqueous mixture (SIRB-001) consisting of 3 Traditional Chinese Medicine(TCM) based herbs; Rheum palmatum L. (Da Huang), Rehmannia glutinosaLibosch (Sheng di huang) and Lonicera Japonica (Jin yin hua) in theratio 1:1:3. SIRB-001 has previously demonstrated promisinganti-psoriatic activity in pre-clinical and clinical studies. SIRB-001was further tested for efficacy in eczema using pre-clinical models,followed by clinical trial. SIRB-001 has demonstrated significantinhibitory effects on secretion of TNF-α, TARC and VEGF in keratinocytes(HaCaT). Considerable downregulation of IL-6 in RAW264.7 cells and IgEin human myeloma cell line-U-266 was observed. SIRB-001 also exhibitedinhibition of JAK-1/JAK-3. SIRB-001 based cream was developed and wasfound to be highly safe in animal studies. Encouraging preclinicalresults paved the path for clinical investigation in 30 subjects witheczematous lesions. The efficacy, safety and tolerance of SIRB-001 creamwas evaluated in a mono-centric, open label study with treatment regimenof twice daily application for 4 weeks. At weekly intervals, EczemaSeverity Index (ESI), Investigator's Global Assessment Severity (IGAS)and IgE levels were determined. SIRB-001 cream demonstrated significant(p<0.001) decrease in ESI, IGAS (d28-d0) and was well tolerated in humanpatients with a good safety profile. Hence, it can be concluded thatSIRB-001 is a highly effective new treatment with a favourable safetyprofile for management of eczema

Treatment methods and medicinal compositions are provided for treatingeczema, and other skin conditions. Certain embodiments involve the useof herbal combinations and combinations of certain herbs, certain herbalextracts and/or certain molecular components of certain herbs, alone orin combination with or supplemental to one or more other known or novelor experimental treatments. Certain embodiments are formulated as ashampoo, conditioner, cream, ointment or other topical scalp or hairtreatment.

Methods and medicinal compositions are provided to treat eczema, and/orother skin conditions such as psoriasis, psoriatic arthritis, or otherinflammatory skin disorders, dandruff including seborrhoeic dandruff,seborrhea, acne, burns, dermatitis including aptoptic dermatitis, warts,keratosis, acne, alopecia, hirsutism or capitis, melanoma ornon-melanoma skin cancer, basal cell cancer (BCC), squamous cell cancer(SCC), scleroderma, dermatofibrosarcoma protuberans, Merkel cellcarcinoma, Kaposi's sarcoma, keratoacanthoma, spindle cell tumors,sebaceous carcinomas, microcystic adnexal carcinoma, Paget's disease ofthe breast, atypical fibroxanthoma, leiomyosarcoma, and angiosarcoma,Marjolin's ulcers, or warts or burns, or another skin ailment, lesion orsore, particularly of the scalp and also for other skin regions affectedby an ailment that is susceptible to topical or hair treatment, as wellas hair fall or hair loss conditions.

In certain embodiments, herbs are advantageously combined as herbalcombinations including Da Huang and Sheng Di Huang. Certain embodimentsalso include Jin Yin Hua. Other embodiments include one or more of MuDan Pi, Di Gu Pi, Xian He Cao, and Chun Gen Pi. Certain embodimentsinclude herbal extracts or molecular components such as emodin, digoxin,and/or other molecules such as aucubin, beta-sitosterol, vanillic acid,rhein, rhapontin and carvacrol. Combinations of these and other herbs,herbal extracts and/or molecules described herein are provided invarious embodiments.

Along with certain combinations of herbs and/or herbal extracts,emotives or molecular components as described herein, a shampoo,conditioner, cream, ointment or other topical eczema treatment inaccordance with certain embodiments may include one or more othercomponents including one or more surfactants and/or co-surfactants,thickening agents, pH adjusters, buffers, aesthetic additives, water,hydro-alcoholic hair serum and/or another dispersive agent, solvent,solubilizer or vehicle, hair-fall or hair-loss control actives,conditioners, preservatives and/or moisturizers and/or vitamins,humectants, one or more cationic polymers, silicone, a suspending agent,perfume and/or other additives that may be consistent with a healthyshampoo, conditioner, cream, ointment or other topical eczema treatment.

A sulphate free eczema shampoo may be formulated with the followingingredients:

An active herbal combination of Da Huang, Sheng Di Huang and Jin YinHua,

aqua (DM water),

PEG 80 Sorbitan Laurate,

Sodium Lauroyl Sarcosinate,

Disodium EDTA,

Niacinamide,

Citric Acid,

Sodium Benzoate, and

Phenoxy Ethanol.

In alternative embodiments, the active herbal combination may include DaHuang and Sheng Di Huang without Jin Yin Hua, or Da Huang and Jin YinHua without Sheng Di Huang, or Sheng Di Huang and Jin Yin Hua without DaHuang, or Da Huang alone with Sheng Di Huang nor Jin Yin Hua, or ShengDi Huang alone without Da Huang nor Jin Yin Hua, or Jin Yin Hua alonewithout Da Huang nor Sheng Di Huang.

In alternative embodiments, a sulphate free eczema shampoo may includePEG-4 Dilaurate, PEG-7 Glyceryl Cocoate, one or more PEG-20 AlmondGlycerides, and/or PEG-25 Hydrogenated Castor Oil instead of or inaddition to PEG 80 Sorbitan Laurate.

In alternative embodiments, an eczema shampoo may include sodium laurylsulphate instead of or in addition to Sodium Lauroyl Sarcosinate,although an eczema shampoo in accordance with these alternativeembodiments would not be sulphate free.

In alternative embodiments, a sulphate free eczema shampoo may includeascorbic acid instead of or in addition to citric acid.

-   -   The sulphate free eczema shampoo may further include one or more        of the following additional components:

CAPB; or

Glycerine, Diethylene Glycol, Propylene Glycol, and/or one or moreCeramides, Oils and/or Butters; or

PEG 4 rapseedamide, Macrogol 200, and/or Polyoxyethylen(4); or

Perfume allergen free and/or any GRAS natural perfume; or

Poly Quat 10, Poly Quat 4, and/or Poly Quat 44; or

PEG 150 Distearate, Antil 120 Plus, and/or Antil 127; or

Olive Leaf Extract;

Or combinations of two or more of these additional components.

A sulphate free eczema shampoo may be formulated with the followingingredients:

An active herbal combination of Da Huang, Sheng Di Huang and Jin Yin Hua(6 mg), aqua (DM water), CAPB, PEG 80 Sorbitan Laurate, Sodium LauroylSarcosinate, Glycerine, PEG 4 rapseedamide, Perfume allergen free,Disodium EDTA, Niacinamide, Poly Quat 10, Citric Acid, PEG 150Distearate, Sodium Benzoate, Phenoxy Ethanol, and Olive Leaf Extract.

In alternative embodiments, the active herbal combination may include DaHuang and Sheng Di Huang without Jin Yin Hua, or Da Huang and Jin YinHua without Sheng Di Huang, or Sheng Di Huang and Jin Yin Hua without DaHuang, or Da Huang alone with Sheng Di Huang nor Jin Yin Hua, or ShengDi Huang alone without Da Huang nor Jin Yin Hua, or Jin Yin Hua alonewithout Da Huang nor Sheng Di Huang.

An eczema shampoo may be formulated with the following ingredients:

An active herbal combination of Da Huang, Sheng Di Huang and Jin Yin Hua(6 mg), Aqua (D M Water), Sodium Laureth Sulphate 70%,Cocomonoethanolamide, Dimethiconol (and)Triethanolamine-Dodecylbenzenesulfonate, Ethylene Glycol Distearate,Sodium Chloride, D-Panthenol, Polyacrylic Acid 940, GuarHydroxypropyltrimonium Chloride, Methylchloroisothiazolinone andMethylisothiazolinone, Sodium Hydroxide, Ethylenediaminetetraacetic acidtetrasodium salt, Tocopheryl acetate and Citric Acid.

In alternative embodiments, an eczema shampoo may include sodium laurylsulphate instead of or in addition to sodium laureth sulphate 70%.

In alternative embodiments, an eczema shampoo may include SLES, SLS,ALES, and/or ALS instead of or in addition to Cocomonoethanolamide.

In alternative embodiments, an eczema shampoo may include glycolstearate and/or glycol stearate SE instead of or in addition to ethyleneglycol distearate.

In alternative embodiments, an eczema shampoo may include Coconut oiland/or avocado oil instead of or in addition to D-Panthenol.

In alternative embodiments, an eczema shampoo may includePolyvinylalcohol (PVA) instead of or in addition to Polyacrylic Acid940.

In alternative embodiments, an eczema shampoo may include Potassiumhydroxide instead of or in addition to Sodium Hydroxide.

In alternative embodiments, an eczema shampoo may include ascorbic acidinstead of or in addition to citric acid.

An eczema shampoo may also include perfume, perfume baby splash and/orany GRAS natural perfume.

An eczema shampoo may also include Cocoamidopropylbetaine instead of orin addition to Cocomonoethanolamide.

A hair lotion for treating eczema may be formulated as follows:

DM Water, an active herbal combination of Da Huang, Sheng Di Huang andJin Yin Hua (6 mg), Propylene Glycol, Glyceryl Monostearate SE, LightLiquid Paraffin, Cetyl alcohol, Stearic acid, Cyclopentasiloxane,Phenoxyethanol & Triethylene glycol, Triethanolamine,Ethylenediaminetetraacetic acid tetrasodium salt and Alkyl Acrylates.

In alternative embodiments, an eczema shampoo may include1,3-Propanediol instead of or in addition to Propylene Glycol.

In alternative embodiments, an eczema shampoo may include white softparaffin instead of or in addition to Light Liquid Paraffin.

In alternative embodiments, an eczema shampoo may include cationic guar,one or more silicones, Honeyquat, and/or one or more polyquats insteadof or in addition to Cetyl alcohol.

In alternative embodiments, an eczema shampoo may include Emulsifyingwax, Beeswax instead of or in addition to stearic acid.

In alternative embodiments, an eczema shampoo may include C13-16isoparaffin (and) C12-C14 isoparaffin (and) C13-C15 alkane instead of orin addition to Cyclopentasiloxane.

In alternative embodiments, an eczema shampoo may include Biotin,methyparaben, carbomer, cetyl alcohol, and/or propylparaben instead ofor in addition to Triethanolamine.

A hair lotion for treating eczema may further include Coconut oil, oliveoil, butter, bacon grease, peanut oil and/or or macadamia oil.

A hair lotion for treating eczema may also include perfume, perfume babysplash and/or any GRAS natural perfume.

A method of treating a scalp condition in accordance with certainembodiments includes applying to the scalp a medicinal compositionformulated as a shampoo, conditioner, cream, ointment or other topicalscalp or hair treatment that comprises a low concentration formula 5-10grams or more of an active herbal combination of Sheng Di Huang, DaHuang and Jin Yin Hua in an example 16 fluid ounce formulation. A highconcentration formula may include 50-150 grams of the active herbalcombination in an example 16 fluid ounce formulation. A mediumconcentration formula may include 10-50 grams of the active herbalcombination in an example 16 fluid ounce formulation.

In certain embodiments, the medicinal composition may include 1%-20% ofthe active herbal combination. In certain embodiments, the medicinalcomposition may include 2.5%-10% of the active herbal combination. Incertain embodiments, the medicinal composition may include 2.5%-5.0% ofthe active herbal combination. In certain embodiments, the medicinalcomposition may include 3.5 wt. % or more of Da Huang. In certainembodiments, the medicinal composition may include 15.4 wt. % or more ofa combination of Da Huang and Sheng Di Huang.

In certain embodiments, the herbal combination may include 2-20 grams ofJin Yin Hua, 2-20 grams of Da Huang and 6-60 grams of Sheng Di Huang. Incertain embodiments, the medicinal composition may include 0.2 wt. %-4wt. % of Jin Yin Hua, 0.2 wt. %-4 wt. % of Da Huang, and 0.6 wt. %-12wt. % of Sheng Di Huang.

In certain embodiments, the herbal combination may include 13.3-60 gramsof Sheng Di Huang, 3.3-15 grams of Da Huang, and 3.3-15 grams of Jin YinHua.

In certain embodiments, the combination of Sheng Di Huang, Da Huang andJin Yin Hua comprises 20 wt. %-80 wt. % of the active herbalcombination. For example, the active herbal combination may include 12wt. %-48 wt. % of Sheng di Huang, 4 wt. %-16 wt. % of Da Huang and 4 wt.%-16 wt. % of Jin Yin Hua.

The active herbal combination may include 18 wt. % or more of Sheng DiHuang, 6 wt. % or more of Da Huang and 6 wt. % or more of Jin Yin Hua.

A medicinal composition is also provided, in accordance with certainembodiments, that is formulated as a shampoo, conditioner, cream,ointment or other topical scalp or hair treatment, comprising an herbalcombination of 13.3 grams or more of Sheng Di Huang and 3.3 grams ormore of Da Huang in a 16 fluid ounce formulation. In certain embodiment,the herbal combination also includes 3.3 grams of Jin Yin Hua.

The medicinal composition may include 0.5 wt. % or more of Da Huang and1.5 wt. % or more of Sheng Di Huang. In certain embodiments, themedicinal composition also includes 0.5 wt. % or more of Jin Yin Hua.

The medicinal composition may include 0.5 wt. %-1.5 wt. % of Da Huangand 1.5 wt. %-4.5 wt. % of Sheng Di Huang. In certain embodiments, themedicinal composition also includes 0.5 wt. %-1.5 wt. % of Jin Yin Hua.

The medicinal composition may include 6 wt. % or less of Da Huang and 18wt. % or less of Sheng Di Huang. In certain embodiments, the medicinalcomposition includes 6 wt. % or less Jin Yin Hua.

The active herbal combination may include 40 wt. % or more of Sheng DiHuang and 10 wt. % or more of Da Huang. In certain embodiments, theactive herbal combination may include 10 wt. % or more Jin Yin Hua.

The active herbal combination within the medicinal composition mayinclude 22.4 wt. % or more, 30 wt. % or more or 60 wt. % or more of thecombination of Sheng Di Huang, Da Huang and Jin Yin Hua.

The active herbal combination may include 15.4 wt. % or more of Sheng DiHuang, 3.5 wt. % or more of Da Huang and 3.5 wt. % or more of Jin YinHua.

The active herbal combination may include 18 wt. % or more of Sheng DiHuang, 6 wt. % or more of Da Huang and 6 wt. % or more of Jin Yin Hua.

Other medicinally active or inactive components may be included incombination with the herbal combinations described herein. The herbalcombination may be administered alone or in combination with orsupplemental to one or more other medicinal treatments.

Referring to FIG. 1, an example of a shampoo in accordance with certainembodiments is illustrated as including nine generalized components.These nine components include a surfactant, a thickening agent, a pHadjuster/buffer, an aesthetic additive, water, a conditioner, one ormore active herbs or herbal extracts or molecules, a preservative andmoisturizers/vitamins. Shampoos in accordance with various alternativeformulations may include fewer than all of these nine components andthey may include other active or inactive components known to thoseskilled in the art as having some advantage when included in a shampooformula or in a medicinal combination for treating eczema, or anotherskin condition such as psoriasis, melanoma, or dermatitis or hair lossor another head or scalp disorder or ailment.

A shampoo in accordance with certain embodiments includes one or moresurfactants that may be known or discovered as being advantageous forcleaning hair with a shampoo. A primary surfactant may be included toprovide flash foam for cleaning the hair by removing dirt and otherimpurities. A secondary surfactant may be included to provide stablefoam and to reduce the harshness of the primary surfactant. A surfactantmay be used that includes a charged, hydrophilic head group and a long,hydrophobic alkyl chain tail. Surfactants are configured to breakmolecular bonds between dirt and hair and to transport the dirt into anaqueous medium to be rinsed free from the hair and scalp. Examples ofsurfactants that may be contained in a shampoo in accordance withcertain embodiments include sodium laureth sulphate, ammonium laurethsulfate, and sodium cocoyl isethionate. Examples of co-surfactantsinclude cocamide MEA and cocoamidopropyl betaine.

A shampoo in accordance with certain embodiments may include athickening or suspending agent. Examples of thickening or suspendingagents that may be contained in accordance with certain embodimentsinclude carbomer and PEG 150 distearate. The thickening agent may beincluded to stabilize the shampoo during storage and/or to prevent thesetting or dumping of pigments and silicone.

A pH adjuster or buffer may be included in a shampoo in accordance withcertain embodiments. An example of a pH adjuster or buffer includescitric acid, tartaric and sodium hydroxide. The pH adjuster or buffer isconfigured to cause the shampoo to be gentle to the skin. A lower pH maycause hair to be compact and to shine and to protect the surfactant fromhydrolysis, and as such, the pH adjuster or buffer may serve to lowerthe pH of the shampoo. However, alternative embodiments include pHadjusters that serve to raise the pH of a shampoo that contains anherbal formula that exhibits an exceptionally low pH.

An aesthetic additive may be included in a shampoo in accordance withcertain embodiments. Examples of aesthetic additives include colorants,opacifiers, UV absorbers, perfumes and natural and artificialfragrances.

One or more conditioners may be included in a shampoo in accordance withcertain embodiments. The one or more conditioners may include a cationicpolymer such as guar hydroxypropyl trimonium chloride. The one or moreconditions may include silicone and/or a silicone emulsion such asdimethiconol, dimethicone, or amodimethicone. The silicone and/orsilicone emulsion may serve to coat the hair and cause the hair tobecome soft, smooth and shiny.

A shampoo in accordance with certain embodiments includes one or moreactive herbs or herbal extracts or emotives that are described inseveral examples herein. These one or more active herbs or herbalextracts serve to promote treatment of eczema, and/or another conditionsuch as psoriasis, dermatitis, melanoma, hair loss and other hair orscalp conditions described herein or understood by those skilled in theart.

A preservative may be included in a shampoo in accordance with certainembodiments. The preservative may be configured to prevent microbialgrowth. Examples of preservatives that may be contained in a shampoo inaccordance with certain embodiments include paraben free, formaldehydedonor free and halogenated free.

A moisturizer and/or one or more vitamins may be included in a shampooin accordance with certain embodiments. Examples include combinations ofD-Panthenol, vitamin E acetate, sodium PCA, glycerine and one or moreamino acids. The moisturizer and/or vitamins may be configured topenetrate into hair shaft, seal cuticles and keep hair moisturized.

A shampoo in accordance with certain embodiments may include ahydro-alcoholic hair serum. Referring to FIG. 2, a hair growth cycleincludes exogen, anagen, catagen and tetogen phases. The anagen phaseinvolves active hair growth, whereby hair follicles regenerate andgenerate pigmented hair shafts. The telogen phase is a resting phase.The catagen phase involves cessation of hair growth and pigmentation,and release of papilla from the bulb. A hydro-alcoholic hair serum maybe configured as a concentrated product that is typically left on thehair for a more extended duration than an ordinary shampoo with atypical shower routine. The hydro-alcoholic hair serum may be configuredto be light and non-sticky on the scalp and as a non-irritant, to belight and non-sticky on the hair, to have little or no effect on hairvolume, to strengthen scalp and DPC, to provide keratinization andcollagen synthesis, to promote hair growth or to control hair fall, orcombinations thereof. For example, the attributes of a hydro-alcoholichair serum in accordance with certain embodiments may assist or promotetreatment of eczema. A hydro-alcoholic hair serum in accordance withcertain embodiments may include water, alcohol, humectant, solubilizer,water-based polymer, scalp conditioner, niacinamide, caffeine andpanthenol. The ratio of alcohol, water and solubilizer may be adjusteddepending of the solubilization power of the active herbal treatmentcomposition.

A method is provided for treating eczema, and/or another disorder suchas psoriasis, dermatitis, melanoma, and/or hair loss. The eczematreatment includes administering to a patient suffering from and/ordiagnosed with eczema. The eczema treatment includes combinations ofcertain herbs, herbal extracts, and/or compounds or molecules extractedfrom certain herbs or herbal extracts. Among these are herbs are one ormore of Da Huang, Sheng Di Huang, and Jin Yin Hua, and/or one or more ofMu Dan Pi, Di Gu Pi, Xian He Cao, and Chun Gen Pi. Particularly studiedamong combinations of these herbs and others described herein includethe three herbs Sheng Di Huang, Da Huang and Jin Yin Hua, threecombinations of two of these three herbs, and formulae including one ofthese three herbs.

In one example, a shampoo formulation may include between 2.5 wt. %-5.0wt. % of a combination of Sheng Di Huang, Da Huang and Jin Yin Hua.

In another example, a shampoo formulation may include between 1.0 wt.%-10.0 wt. % of a combination of Sheng Di Huang, Da Huang and Jin YinHua.

In another example, a shampoo formulation may include between 1.0 wt.%-15.0 wt. % of a combination of Sheng Di Huang, Da Huang and Jin YinHua.

An eczema regimen may include combinations of applications of shampoo,cream and/or lotion to affected scalp or other skin areas.

Administration in a treatment regimen of certain combinations with oneor two or more of these herbs serve to treat hair and scalp conditionsas provided in accordance with embodiments described herein. Specificembodiments include advantageous combinations of Da Huang and Sheng DiHuang, as indicated below and in U.S. patent application Ser. No.13/018,435, which is incorporated by reference, as well as withcombinations including Jin Yin Hua with Da Huang and/or Sheng Di Huang.Further embodiments include combinations of DaHuang, Sheng Di Huangand/or Jin Yin Hua with one or more of Mu Dan Pi, Di Gu Pi, Xian He Cao,and/or Chun Gen Pi.

Further embodiments include combinations of beta-sitosterol or sawpalmetto, or both, with Da Huang, Sheng Di Huang and/or Jin Yin Hua, andone or more of Mu Dan Pi, Di Gu Pi, Xian He Cao, and/or Chun Gen Piand/or one or more other herbs or molecules described herein. Furtherembodiments include herbal combinations of one or more of Sheng DiHuang, Da Huang and Jin Yin Hua with combinations of one or more ofemodin, digoxin, beta-sitosterol, saw palmetto, aucubin, rhein,rhapontin, vanillic acid, carvacrol or other herbs or moleculesdescribed herein or as understood by those skilled in the art. Otherembodiments include combinations including one or more of more of Mu DanPi, Di Gu Pi, Xian He Cao, and/or Chun Gen Pi.

Contained within each of the seven herbs are several molecularconstituents. Observed reductions of psoriatic inflammation and otherstudied effects owing to a treatment regimen of periodic shampooing withan herbal formula in accordance with the embodiments can be as a resultof various combinations of active molecules contained in Da Huang, JinYin Hua and/or Sheng Di Huang, and of combinations of the herbsthemselves.

A method of treating Melanoma is also provided including administeringto a patient diagnosed with Melanoma a treatment regimen that includesperiodic application to the scalp of an herbal formula including ShengDi Huang, Da Huang or Jin Yin Hua, or combinations thereof, along withthe administration of Aldesleukin (Proleukin), Dacarbazine, Ipilimumab(Yervoy), or Peginterferon-alpha or combinations thereof.

Another method of treating Melanoma is provided including administeringto a patient diagnosed with Melanoma a treatment regimen that includesperiodic applications to the scalp of an herbal formula including ShengDi Huang, Da Huang or Jin Yin Hua, or combinations thereof, along withthe administration of Paclitaxel, Cisplatin, Carboplatin, Vinblastine,Vincristine or Vindesine, or combinations thereof. The treatment regimenmay also include Aldesleukin (Proleukin), Dacarbazine, Ipilimumab(Yervoy) or Peginterferon-alpha, or combinations thereof.

Another method of treating Melanoma is provided including administeringto a patient diagnosed with Melanoma a treatment regimen that includesperiodic application to the scalp of Sheng Di Huang, and Da Huang, orSheng Di Huang, Da Huang and Jin Yin Hua in combination with orsupplemental to periodic doses of Fluorouracil or another pyrimidineanalog, Pembrolizumab, methotrexate, Nivolumab, Pidilizumab, one or moremonoclonal antibodies, one or more drugs that target PD-1 receptors orPD-1 inhibitors, and/or with radiation treatments such as protontherapies for treating choroidal melanomas, endolymphatic radio-isotopeinfusion or other radiation therapy such as external beam radiotherapyor brachytherapy, topical chemotherapy, e.g., with imiquimod or5-fluorouracil, and cryotherapy, photodynamic therapy,electrodesiccation, curettage, Mohs' micrographic surgery.

A method of treating eczema is provided including administering to apatient diagnosed with eczema a treatment regimen that includes periodicdoses, such as by application to the scalp by shampooing and rinsing orotherwise, of a combination of Sheng Di Huang and Da Huang and/or acombination of Sheng Di Huang, Da Huang and Jin Yin Hua and/orbeta-sitosterol, saw palmetto, emodin, digoxin, aucubin, rhein,rhapontin, vanillic acid, carvacrol, or combinations thereof, along withConsentyx (secukinumab) or other IL-17 inhibitor, IL-12, IL-23 or TNF-αinhibitors, Otezla (apremilast) or other phosphodiesterase-4 inhibitor,Humira, Stelara, alefacept, methotrexate, cyclosporine,hydroxycarbamide, fumarates such as dimethyl fumarate, topicalretinoids, monoclonal antibodies such as infliximab, adalimumab,golimumab, or certolizumab pegol, a proteasome inhibitor, Donavan'ssolution, vitamin A, D, D₃ or related analogs or synthetic forms,paricalcitol, calcipotriol, fluocinonide, dithranol, decubol, psoralen,acitretin, coconut oil, mineral oil, balnoetherapy or baths in the deadsea or in formulations of dead sea or dead sea-like minerals, salts,emollients, or oils, fish oil or gluten-free dieting, UV light therapy,dermabrasion, laser therapy, cryotherapy, or combinations of two or moreof these or combinations of these with other treatments or medicationsor compositions described herein.

A method of treating or preventing hair loss is also provided includingperiodically applying to the hair and scalp such as by shampooing anherbal combination of Sheng Di Huang and Da Huang and/or a combinationof Sheng Di Huang, Da Huang and Jin Yin Hua, and/or a combination of oneor more of Sheng Di Huang, Da Huang or Jin Yin Hua or other herbsdescribed herein or as understood by those skilled in the art with sawpalmetto or beta-sitosterol or a combination of beta-sitosterol and sawpalmetto, and/or one or more of emodin, digoxin, aucubin, rhein,rhapontin, vanillic acid, or carvacrol, or combinations thereof.

Another method of treating hair loss is provided including periodicapplication to the hair and scalp of an herbal formula including one ormore of Da Huang, Sheng di Huang or Jin Yin Hua formulated with orsupplemental to a treatment that includes minoxidil, finasteride,dutasteride, anthralin, or an anabolic steroid or corticosteroidtreatment, or an acne treatment, or a hormone replacement therapy, or anoral contraceptives or antiandrogen such as spironolactone or flutamide.

A method of treating glioblastoma is provided including periodicallyapplying to the scalp such as by shampooing or application of a cream,or by oral administration or sub-dermal injection or IV, of acombination of Sheng Di Huang and Da Huang and/or a combination of ShengDi Huang, Da Huang and Jin Yin Hua and/or beta-sitosterol, saw palmetto,emodin, digoxin, aucubin, rhein, rhapontin, vanillic acid, carvacrol, orcombinations thereof, along with TEMODAL, methotrexate, betamethasone,temozolomide, or Avastin, or combinations thereof.

Methods of relieving side effects of medical treatment for a hair orscalp condition, improving quality of life of a patient undergoingmedical treatment, inhibiting certain growth factors or markers,increasing a therapeutic index of therapeutic compounds for treating ahair or scalp disorder, condition or disease, and modulatinghematological or immunological activity for the treatment of a disease,are also provided, including administering a combination of Sheng DiHuang, Da Huang and Jin Yin Hua, along with one or more of thefollowing: Vincristine Sulfate, Cyclophosphamide, DoxorubicinHydrochloride, Methotrexate, Cyclosporine, Clofarabine, Cytarabine,Dasatinib, Daunorubicin Hydrochloride, Gleevec (Imatinib Mesylate),Nelarabine, Oncaspar (Pegaspargase) Doxorubicine, Daunorubicin,Vincristine Paclitaxel, Cisplatin, Carboplatin, Vinblastine,Vincristine, Vindesine, Aldesleukin (Proleukin), Dacarbazine, Ipilimumab(Yervoy), Talidomid, Revlomid, Gleevac, Dasatinib, Sprycel, Nilotinib,Tasigna, Hydroxyurea, Hydrea, Valkade, Peginterferon-alpha, an RCHOPcombination, or an ABVD combination, Folfiri q Folfox, Cisplatinum,etoposide, mitomycin C, vindesine Taxotere, Paclitaxel, Docetaxel,Mitoycin C, Doxorubicin, Mitozantrone, vinblastine, Etoposide,Estramustine Phosphate, cyclophosphamide, doxorubicin, Adriamicin,fluorouracil, 5fu, Taxol, Carboplatinum, Erbitux, TEMODAL, temozolomide,or Avastin, or combinations thereof.

Advantageous effects of a shampoo or other hair or scalp treatmentregimen may be bolstered by further combinations with active ingredientsand/or by one or more buffer molecules or one or more molecules servingas some helpful vehicle for the active molecules. The chemistries andpharmacologies of certain herbs and molecules that may be included in ashampoo containing an herbal formula are described in examples inaccordance with certain embodiments.

In certain embodiments, certain parts of the herbs are used such as theroots, stems, leaves, husks, branches, barks, sap, or kernels orcombinations thereof. For example, for Da Huang at least the root may beused. For Sheng Di Huang, at least dried root tuber may be used. For JinYin Hua, at least dried flower may be used. For Mu Dan Pi, at leastdried root bark may be used. For Di Gu Pi, at least dried root bark maybe used. For Xian He Cao, at least dried aerial part of Agrimonia PilosaLedeb may be used. For Chun Gen Pi, at least dried bark of the root orstem may be used.

In certain further embodiments, combinations of Sheng Di Huang and DaHuang, or two or more of the three herbs including Jin Yin Hua, orcombinations of the seven herbs including Mu Dan Pi, Di Gu Pi, Xian HeCao and/or Chun Gen Pi with any one or more of the three herbs, may becombined with any one or more of the following additional eleven herbsincluding Zi Cao, or radix arnebiae (arnebia root) or radix lithospermi(gromwell root), Xuan Shen, or radix scrophulariae (figwort root), ShiGao or gypsum fibrosum (gypsum), Bai Shao, or radix paeoniae alba (whitepeony root), Chi Shao or radix paeoniae rubra (red peony root), Hong Huaor flos carthami (safflower), Da Qing Ye or folium isatidis (woad leaf),Qing Dai or indigo naturalis (natural indigo), Bai Zhu or rhizomaatractylodis macrocephalae (largehead atractylodes rhizome), and Shi Weior folium pyrrosiae (shearer's pyrrosia leaf), Rou Gui or cortexcinnamomi (cinnamomum bark). An advantageous herbal shampoo formula mayalso include beta-sitosterol, saw palmetto, emodin, digoxin, aucubin,rhein, rhapontin, vanillic acid, or carvacrol, or combinations thereof,or any of the other molecules described herein or as may be understoodto those skilled in the art.

Da Huang Radix Et Rhizoma Rhei (Rhubarb) Chemistry

Da Huang contains free antraquinones, anthraquinone glycosides, andbianthrones. Among the free antraquinones contained within Da Huang arealizarin, aloe emodin, chrysophanol, citreorosein, emodin, laccaic acidD, physcion, and rhein. Among the anthraquinone glycosides containedwithin Da Huang are1,8-dihydroxy-3-methylanthraquinone-1-O-β-D-glucoside (Palmatin), aloeemodin 1′-O-β-D-glucopyranoside, aloe emodin 1-O-β-D-glucopyranoside,chrysophanol 1-O-β-D-glucopyranoside (chrysophanein), chrysophanol8-O-β-D-glucopyranoside, emodin 1-O β-D-glucopyranoside, emodin3-O-β-D-glucopyranoside (Glucoemodin), emodin 8-O-β-D-glucopyranoside,physcion 1-O-β-D-glucopyranoside, physcion 8-O-β-D-gentiobioside,physcionin, and rhein 1-O-β-D-glucopyranoside. Among the bianthronescontained in Da Huang are aloe emodin bianthrone, chrysophanolbianthrone, palmidins A-C, rheidins A-C, sennidins A, B and C andsennosides A-F.

Da Huang also includes other compounds including2-(-2-hydroxy-propyl)-5-methyl-7-hydroxy-chromone,2-(-2-hydroxypropyl)-methyl-7-hydroxy-chromanone,2,5-dimethyl-7-hydroxychromone,2-methyl-5-carboxymethyl-7-hydroxychromone, 3 napthalenes,3,5,4′-trihydroxystilbene 4′-β-D-(2″-O-galloyl)-glucopyranoside,3,5,4′-trihydroxystilbene 4′-O-β-D-(6″-O-galloyl)-glucopyranoside,4′-O-methylpiceid, Rhapontin, Rheinosides A-D, Stilbene gakkates3,5,4′-rtihydroxystilbene 4′-O-β-D-glucopyranoside, Stilbene piceid andTannins.

Pharmcalogy Purgative Effect of Da Huang

Da Huang is well known as a purgative agent. The active constituents arethe combined anthraquinones, especially sennosides. The content ofsennosides correlates with the purgative activity of rhubarb.

TABLE 1 illustrates the oral purgative ED₅₀ values of the anthraquinonesAnthraquinones ED₅₀ (mg/kg) Anthraquinones ED₅₀ (mg/kg) Sennoside A 13.5Aloe-emodin-8-glycoside 71.6 Sennoside B 13.9 Emodin monoglucoside 103.6Sennoside C 13.3 Aloe-emodin 59.6 Sennoside D 13.8 Rhein 97.5 SennosideE 13.5 Emodin >500 Sennoside F 16.1 Physcion >500 Rhein-8-glucoside 20.0Chrydophanol >500

Studies on the mechanisms of action found that sennosides actpredominantly on the large intestine. The most potent purgative activitywas obtained from the rhubarb extract or Sennoside A by gastricaladministration and from sennidin by intravenous route. Inhibition of theintestinal flora in mice with chloramphenicol significantly decreasedthe activity prosthetic sugar group of the anthraquinone glycosidesprevented the anthrone from being oxidized before they are transportedinto the large intestine and hydrolysed by the bacterial enzyme intofree sennidins. It was found that sennosides are hydrolysed by microbialβ-glycosidase in a stepwise fashion to the corresponding sennidins via8-monoglycosides. The resulting metabolites sennidins were furtherreduced, possibly by a reductase bound to cell membranes of intestinalbacteria, to rheinanthrone as the purgative principle.

Ligation of the junction of the large and small intestines failed toprevent anthraquinone glycoside producing a purgative effect in thelarge intestine. Oral administration of rhubarb started to produceeffect 6-8 h later. These results suggest that there is also a largepart of anthraquinone glycosides absorbed in the small intestine andtransformed in the liver before they act on the pelvic plexus andproduce peristalsis and purgation.

On the other hand, small doses (0.05-0.3 g by oral administration)rhubarb caused constipation because of its high content of tannins. Theconstipation effect can be prevented by decocting rhubarb together withHuang Lian (Rhizoma Coptidis). This is because the tannins andberberine, the main constituent of Huang Lian, form gelatinousprecipitates during decoction.

Antimicrobial Effect of Da Huang

Tested by mixing virus with dilutions of aloe emodin for 15 min at 37°C., herpes simplex virus type 2 and type 3, varicella-zoster virus,pseudorabies virus, influenza virus were inactivated. Electronmicroscopic examination of the virus demonstrated that the envelopeswere partially disrupted, indicating that it is directly virucidal toenveloped viruses. Emodin and rhein showed antiviral activity againsthuman cytomegalovirus (HCMV) strain AD-169. When tested against aganciclovir-resistant strain of HCMV, the EC₅₀ value for rhein wassuperior to the value obtained for the AD-169 strain. The aqueousextract of R. palmatum inhibited hepatitis B virus (HBV) polymeraseactivity and to bind hepatitis B virus surface antigen (HBsAg).Intravenous dose of 50 mg/kg of the extract to duck hepatitis B virus(DHBV) carrier ducklings showed antiviral activity against DHBV usingserum DHBV DNA level and DHBV DNA polymerase activity as antiviralindicators.

Rhubarb exhibited inhibition against staphylococci, Streptococcushemolyticus, Corynebacterium diphtheriae, Bacillus subtilis, B.brucellosis, B. mycoides, B. smegatis, Mycobacterium graminis, Yerseniapestis, Salmonella tophi, S. paratyphi, Shigella dysenteriae andNeisseria gonorrhea. Staphylococci and Neisseria gonorrhea were mostsensitive to the herb. The main antibacterial components were theanthraquinone derivatives with the structure of1,9-dihydroxyanthraquinone. 3-Carboxyrhein, hydroxyaloe-emodin andhydroxyemodin showed the most potent antibacterial activity. Thebacteriostatic concentrations of rhein, emodin and aloe-emodin againststaphylococci, streptomycin, Corynebacterium diphtheria, Bacillussubtilis, B. anthracis and Salmonella tophy were mitochondrialrespiratory chain of microorganisms. Respiration of Staphylococcusaureus was strongly inhibited by emodin, aloe-emodin and rhein. Rhein,emodin and rhein specifically interfered with the redox function NADHdehydrogenase.

The aqueous, ethanolic and ether extracs of rhubarb are also antifungalagainst many pathogenic fungi, including Achorion schoenleini,Trichopphyton concentricum, T. violaceum, T. gypsum, Nocardia asteroids,Epidermophyton flocosum and Sporotrichum schenckii. The decoction ofrhubarb exhibited inhibition against the influenza virus. The minimaleffective dose in chicken embryo in vitro and semi in vivo was 5 mg perembryo.

Antineoplastic and Antimutagenic Effects of Da Huang

Intraperitoneal administration of 75 mg/kg of emodin produced a 45%inhibition against the mammary carcinoma of mice. The inhibition ratesof 5 mg/kg of rhein and emodin against murine melanoma were 76% and 73%.Rhein, emodin and aloe-emodin inhibited murine leukemia P₃₈₈ in vivo,increasing the survival time and decreasing the ascites volume. Theyalso inhibited Ehrlich ascites carcinoma, emodin was a strong inhibitorof respiration in Ehrlich ascites carcinoma cells, with an ED₅₀ of 20μg/ml. Cellular respiration in leukemia L₁₂₁₀ cells was also inhibited.Palmatin, Cysophanein and physcionin also exhibited moderate cytotoxicactivity against several types of carcinoma cells.

The extract of the herb from R. palmatum and emodin induced adose-dependent decrease in the mutagenicity of benzo(a)pyrene [B(a)P,],2-amino-3-methylimidazo(4,5-f) quinoline (IQ) and3-amino-1-methyl-5H-pyrido(4,3-b)indole (trp-P-2) in Salmonellatyphimurium TA98. It was further found that emodin reduced mutagencityof IQ by direct inhibition of the hepatic microsomal activation and notby interaction with proximate metabolites of IQ and/or by modificationof DNA repair processes in the bacterial cell. Emodin also markedlydecreased the mutagenicity of 1-nitropyrene (1-NP) in a dose dependantmanner in Ames-microsomal test with S. typhimurium TA98 and thegenotoxicity in SOS chromotest with E. coli PQ37. Furthermore, emodinsignificantly inhibited the formation of 1-NP DNA adducts in S.typhimurium TA98. The results suggest that emodin acts as a blockingand/or suppressing agent to reduce the direct-acting mutagenicity of1-NP.

Hemostatic Effect of Da Huang

Rhubarb is also in TCM as a hemastatic agent. The hemastatic activityhas been proved experimentally and clinically. Rhubarb is effective forboth external and internal hemorrhage. It was effective in the treatmentand prevention of experimental gastric bleeding and ulcer formation inrats. Significant therapeutic effects of the powdered rhizome of R.palmatum in the treatment of gastrointestinal bleeding were alsoreported. It reduces coagulation time and the permeability and fragilityof capillaries. It increases fibrinogen and promotes bone marrow toproduce platelets.

Immunosuppressive Effect of Da Huang

Emodin at 3×10⁷-3×10⁻⁴ M dose-dependently suppressed the responses ofhuman mononuclear cells to phytohemagglutinin and mixed lymphocytereaction. It was further found that after exposure to emodin (10⁻⁶ M)the production of interleukin-1 (IL-1) and interleukin-2 (IL-2) and theexpression of IL-2 receptor were all decreased, Emodin may be a newtemplate for the development of better immunosuppressive agents for useagainst transplantation and autoimmune disease.

Choleretic Effect of Da Huang

Rhubarb can stimulate construction of the gallbladder and relax Oddi'ssphinctercan, thus promoting bile secretion. It also increases thecontents of bilirubin and bile acid.

Other Effects of Da Huang

Oral administration if emodin and rhein provoked marked diuretic,natriuretic and kaliuretic effects in rabbits. Oral administration ofrhubarb increased urinary excretion of sodium and potassium, alkalizingurine to a pH value as high as 8.4. Rhubarb also inhibits the activitesof pepsase, trypsase, pancreatic amylase and pancreatic lipase. Itlowers blood pressure and blood cholesterol levels. Rabbits with feverinduced by subcutaneous injection of the pneumococci responded withreduced temperature after oral administration of the decoction ofrhubarb.

Intraperitoneal administration of 15 mg/kg of emodin exhibitedantiinflammatory activity against carrageenin-induced pedal inflammationin rats. In the same dosage it also showed antiulcerative activityagainst pylorus-ligated, aspirin and immobilization stress-inducedgastric ulcer in rats. It decreased acid and pepsin output and augmentedmucus secretion in terms of total carbohydrate:protein ration in thegastric juice of aspirin treated pylorusligated rats, indicating thatthe antiulcerative effect of emodin may be due to this effect on gastricsecretion.

Mu Dan Pi Cortex Moutan (Paeony Bark) Chemistry

Mu Dan Pi contains Apiopaeonoside, Benzoyloxypaeoniflorin,Benzoylpaeoniflorin, Galloyloxypaeoniflorin, Galloypaeoniflorin,Mudanpiosides A-F, Oxypaeoniflorin, Paeconoside, Paeoniflorin,Paeonisuffral, Paeonisuffrone, Paeonol, Paeonolide, Pentagalloyglucose1,2,3,4,6, and Suffruticosides A-E

Pharmacology Antimicrobial Activity of Mu Dan Pi

The decoction of the root bark exhibited a strong antibacterial activityin vitro against the following bacteria: Bacillus subtilis, Escherichiacoli, Salmonella typhi, S. paratyphi, Protues vulgaris, Pseudomonasaeruginosa. Staphylococcus aureus, Strephtococcus hemolyticus,Diplococcus pneumoniae and Vibrio cholerae. Paeonol was one of theantibacterial components; its MIC values were 1:2000 againstStaphylococcus aureus, 1:1500 against Bacillus subtilis and Escherichiacoli.

Anti-Inflammatory Effect of Mu Dan Pi

The 70% methanolic extract of the root bark inhibited rat paw swellinginduced by carrageenin. Paeonol was found active in inhibiting rat pawswelling induced by dextran, acetic acid or carrageenin. It alsoinhibited the increase of intra-abdominal capillary permeability of miceand cutaneous capillary permeability of guinea pigs caused by aceticacid or 5-HT. On the other hand, the water soluble fraction free frompaeonol as well as the glycoside fraction also exhibited a significantinhibitory action on rat paw edema due to carrageenin. The water-solublefraction was also effective in either preventing or treatingadjuvant-induced arthritis in rats. The methanolic extract, theglycosidic fraction and paeonol inhibited blood platelet aggregation.ADP- or collagen-induced human plateet aggregation was inhibited bypaeonol. The formation of thromboxan B₂ was also inhibited but theformation of 12-hydroxy-5,8,10,14-eocpsatetraenoic acid fromC-arachidonate was stimulated. Besides, paeonol inhibited the formationof prostanoids such as prostaglandins and thromboxanes fromC-arachidonate in rat peritoneal macrophages. Thus the anti-inflammatoryaction of the root bark was related to the inhibitory effects of paeonolon prostanoid synthesis.

Hypotensive Effect of Mu Dan Pi

The blood pressure of dogs with essential or renal hypertension wassignificantly reduced after oral administration of 5 g/kg of thedecoction of the root bark for 5 days and 10 g/kg for two more days. Theblood pressure of dogs with renal hypertension was also lowered afteroral of 10 g/kg of the decoction free paeonol for 10 days. Oraladministration of 0.5-1.0 g/kg of paeonol also produced hypotensiveaction renal hypertensive dogs and rats.

Effect on the CNS of Mu Dan Pi

Intraperitoneal or oral administration of paeonol decreased thespontaneous activity of mice, antagonized caffeine-induced hyperactivityand prolonged cyclobarital-induced sleep. At higher doses, paeonolcaused disappearance of the righting reflex in mice. It also antagonizedconvulsions due to cardiazol, strychnine, nicotine and electric shock.Furthermore, paeonol was found to have antipyretic and analgesicactivities. Paeonol decreased the body temperature of normal mice andthe mice with typhoid and paratyphoid vaccine-induced fever. Oraladministration of paeonol produced an analgesic effect against aceticacid-caused writhing and tail pain by pressing in mice.

Effect on Obesity of Mu Dan Pi

The aqueous extract of the herb was given as drinking water at theconcentration of 0.5% to (SLN×C₃H/He) F₁ obese mice between 3 and 32weeks of age. The treatment resulted in a significant decline,particularly in males, in food intake and in the Lee index, An index ofobesity, and furthermore an increase in glucose tolerance. Nosignificant difference was observed between the experimental and thecontrol groups in the serum free fatty acid levels. There was littledifference between groups in the weights of heart, liver, lung, spleenand major endocrine organs in both sexes and in the pattern of oestrouscycles in females, There results indicate that the herb protects againstobesity, especially in males, at least partly by a decrease in foodintake and an increase in glucose metabolism.

Other Effects of Mu Dan Pi

Paeonol exhibited anticholinergic and antihistaminic actions on isolatedileum of mice and guinea pigs. It also prevented stress ulcer in miceand inhibited gastric secretion in rats and spontaneous motility of ratuterus in situ. The extract of the root bark and paeonol were also ofantimutagenic activity. They decreased the frequency of mutationsinduced by 4-nitroquinoline 1-oxide in Escherichia coli WP2s.

Sheng Di Huang Radix Rehmanniae (Chinese Foxglove Root) Chemistry

Sheng Di Huang contains4-(α-L-rhamnopyranosyloxy)-3-methoxybenzoylajugol, Aceutoside, Ajugol,Aucubin, Campesterol, Castanosides A and F, Catalpol, digoxinEchinacoside, E-feruloylajugol, Isoacetoside, Jioglutoside A and B,Jionosides A&B, Leonuride, Mannitol, Melittoside, p-courmaroylagujol,p-hydorxybenzoylajugol, Purpureaside C, Rehmaglutins A, RehmaionosidesA-C, Rehmanniosides A-D, Rehmapicroside, Vanilloylajugol,Z-feruloylajugol, and β-sitosterol,

Pharmacology Effects on Adrenocortical Function and Cortisol Metabolismof Sheng Di Huang

The herb was able to stop the decrease of plasma corticosteroneconcentration due to administration of dexamethasone and prevent theadrenal cortex from atrophy. The corticosterone level in rabbitsreceiving dexamethasone was increased at week 4 and week 6 when the herbwas concurrently applied. No morphological changes were observed in thepituitary gland and adrenal cortex of rabbits receiving concurrenttreatment of dexamethasone and the herb. A single large dose (3 g/kg) ofthe root or together with two other herbs Zhi Mu (Rhizoma Anemarrhenae)and Licorice (0.9 g/kg each) given orally antagonized the inhibitoryeffect of dexamethasone on the pituitary-adrenal system of rabbits,thereby increasing plasma corticosterone level.

This mixture also antagonized the inhibitory action of dexamethason onthe early morning cortisol secretion peak in 12 normal subjects astested in diurnal dexamethason suppression test. The crude extract (8mg) of the root, when incubated with the liver sections of rabbits,protected cortisol from being reduced on the double bond between C₄ andC₅, and the ketone at C₃ and being degraded of the hydroxyl groups atC₁₇ and C₂₁, and the ketone at C₂₀, thus delaying the metabolism ofcortisol in the liver. When the herb was used simultaneously withexogenous adrenocortical hormones, plasma cortisol could still be keptat a nearly normal level. The mechanism is believed to be a kind ofcompetitive effect which influenced the binding of cortical hormone tothe receptors and affected the uptake of corticosteroid hormone by theliver cells, thereby slowing down the catabolism of cortisol.

Cardiovascular and Diuretic Effects of Sheng Di Huang

The effects of the herb on the heart were largely dependent on doses.There was no obvious cordial activity at 0.1 or 0.5% concentration. At1% concentration, cordial effect was observed in the isolated perfusedfrog heart. This action was more obvious in weak heart. Whenconcentrations were increased to 2-5%, the heart was inhibited. Itseffects on blood pressure was also dose-dependent. In an experiment withperfused vessels, 1-3% of the extract caused vasoconstriction while at5% vasodilation.

Intravenous injection of 2.5 ml of the root extract produced a diureticeffect in anesthetized dogs. This action may be related to the cordialand renal vasodilation activities.

Effect on Blood Glucose of Sheng Di Huang

Results in animal experiments have been inconsistent on the effect ofthe herb on blood glucose. Hypoglycemic effects of alcoholic extract ofthe root at a subcutaneous dose of 2 g/kg or an oral dose of 4 g/kg inrabbits were reported by early researchers. The result obtained from thesubcutaneous injection was more significant: the blood sugar wasdecreased to the lowest level 4 h after medication. Subcutaneousadministration of the alcoholic extract to rabbits also inhibited theprolonged hyperglycemic effect elicited by carbohydrates from the rootof Codonopsis pilosula (Dang Shen). Intramuscular administration of 20 gof the same extract also suppressed and prevented epinephrine-inducedhyperglycemia in rabbits. Other studies, however, reported that theaqueous or alcoholic extract could only reduce the blood glucose ofnormal rabbits and was not effective in hyperglycemia due toepinephrine. But there were also reports that the herb had no effect onthe normal blood glucose level of rabbits. The decoction or theethanolic extract at 6 g/kg had no effect on the normal blood glucosemeasurements of rabbits within 6 h of medication. Subcutaneousadministration of 20 g/kg of the same agents also failed to antagonizeepinephrine-induced hyperglycemia in rats. More recently, a weakhypoglycemic activity of rehmannioside D in spontaneous diabetic micewas reported.

Antiinflammatory and Immunosuppressive Effects of Sheng Di Huang

Formaldehyde-induced edema of rat paws subsided after oraladministration of the decoction or alcoholic extract at the daily doseof 10 g/kg for 5 days. However, another report claimed that only thedecoction and not the alcoholic extract had a significantanti-inflammatory activity. At the oral dose of 100 mg/kg, jionoside Band acetoside produced 36% and 18% suppression of hemolytic plaqueforming cells in the spleens of mice. In the same test conditions,intraperitoneal dose of 30 mg/kg of cyclophosphamide had a 52.5%suppression.

Hemostatic Effect of Sheng Di Huang

The coagulation time in rabbits was reduced after giving the yellowneedle crystal obtained from the ethanolic extract of the root.Intraperitoneal administration of 10 g/kg of the decoction or ethanolicextract, or oral administration of the charred herb shortened thebleeding time in mice with tail wounds.

Effect on Hemorheology of Sheng Di Huang

The effects of the herb on the hemorheology of inflammatory, thromosicand intact animals were examined. Oral administration of 200 mg/kg ofthe 50% ethanolic extract of the herb inhibited the reduction offibrinolytic activity erythrocyte deformability, the decrease inerythrocyte counts and the increase in connective tissue of the thoracicartery in a chronic inflammatory model, adjuvant-induced arthritis.However, it was ineffective on the development of edema in the arthriticrats and on acute and chronic inflammation. In addition, the extractinhibited the reduction of erythrocyte deformability but not thedecrease of coagulative factors in a thrombosic model, endotoxin-induceddisseminated intravascular coagulation (DIC). It also exhibited apromoting effect on erythrocyte deformability and fibrinolytic activityin intact rats. There results suggests that oral administration of theextract can prevent an inducement of impediment in the peripheralmicrocirculation of various chronic diseases through the improvement ofhemorheology.

Other Effects of Sheng Di Huang

Antiradiation, antifungal and antihepatotoxic activities have also beenobserved with extract of the root in animals. The 100% injectionsolution of the root given intraperitoneally at 1 ml daily for 6 daysmitigated platelet damage in rats caused by 600 rad of γ-irradiation.The aqueous extract of the root inhibited intro fungi mentagrophyton.Microsporum gypseum and M. audouini. The decoction of the root showedprotective effect in mice against CCI-caused liver intoxication. Oral orintraperitoneal administration of 10 g/kg of the decoction or thealcoholic extract potentiated the hypnotic effect of pentobarbitalsodium. Intraperitoneal dose of 20 g/kg of the decoction or thealcoholic extract protected mice from hypobaric hypoxia.

Jin Yin Hua Flos Lonicerae (Honeysuckle Flower) Chemistry

Jin Yin Hua contains 2,6,6-trimethyl-2-vinyl-5-hydroxytetrahydropyran,Benzyl-alcohol, Carvacrol, Cis andtrans-2-methyl-2-vinyl-5-(α-hydroxyisopropyl)-tetrahydrofuran,Epivogeloside, Eugenol, Geraniol, Hex-1-ene, Hex-3-en-1-ol,Isochlogogenic acid b+c (two isomers of 3,4-dicaffeoyl quinic acid),Isochlorogenic acid a (3,5-dicaffeoyl quinic acid), Linalool, Loganin,Lonicerin, Lonicerin, Luteolin, Methylcaffeate, Pinene, Saponins witholeanolic acid, Secologanin dimethylacetal, Secoxyloganin, sweroside,Vanillic acid, Venoterpin (gentialutine), Vogeloside, α-terpineol, andβ-phenylethyl alcohol

Pharmacology Antimicrobial Activities of Jin Yin Hua

The extracts of both the flower and vine inhibited in vitro thefollowing bacteria Staphylococcus aureus, Streptococcus hemolyticus,Escherichia coly, Shigella dysenteriae, Vibrio cholera, Salmonellatyphi, S. oaratyphi, Diplococcus pneumoniae, Neisseria meningitides,Pseudomonas aeruginosa and Mycobacterium tuberculosis. It alsopotentiated the action of penicillin against the drug-resistantStaphylococcus aureus. Chlorogenic acid and isochlorogenic acid arebelieved to be the chief antibacterial components of the flower.Luteolin also showed an antibacterial activity. More than half of themice receiving the LD dose of Pseudomonas aeruginosa or its endotoxinsurvived after given 7.5 g/kg of the injection solution of the flower byintraperitoneal administration. Intravenous administration of 6 g/kg ofthe distillate of the flower was also therapeutically effective inrabbits poisoned by the endotoxin of Pseudomonas aeruginosa.

Antifunal activity was observed with the aqueous extract of the floweragainst Microsporum ferrugineum and Nocardia asteroids. In the monolayerprimary culture of the epithelial cells of human embryonic kidney, thedecoction of the flower inhibited influenza virus, ECHO virus and herpesvirus.

Anti-Inflammatory Effect of Jin Yin Hua

Intraperitoneal administration of 0.25 g/kg of the flower inhibitedcarrageenin-induced paw edema in rats. Given twice a day at 8 g/kg for 6days by Intraperitoneal injection, the extract of the flower showedantiexudative and antihyperplastic effects on croton oil-inducedgranuloma. Intraperitoneal administration of the injection solutionincreased the phagocytic activity of the inflammatory cells in mice. Thedecoction diluted to 1:1280 concentration was still able to promoteleukocytic phagocytosis.

Central Stimulant Effect of Jin Yin Hua

Oral administration of chlorogenic acid produced central stimulation inmice and rats in experiments using electric shock and revolving cage;the potency of the central stimulation was ⅙ that of caffeine. Noaddictive or synergistic action was observed when they were usedtogether.

Antilipemic Effect of Jin Yin Hua

Oral administration of 2.5 g/kg of the flower reduced the intestinalabsorption of cholesterol and the plasma cholesterol level. In vitroexperiments showed that the flower conjugated with cholesterol.

Other Effects of Jin Yin Hua

Intraperitoneal administration of an aqueous-ethanolic extract of theflower of L. japonica to mice on day 8 after mating decreased pregnancyin the test animals dose-dependently. Intrauterine and intra-amnioticadministration of the extract killed the fetuses in dogs and causedabortion in monkeys, respectively. The extract of the flower exhibited amild prophylactic effect against experimental gastric ulcer in rats whengiven orally. Large oral doses of chlorogenic acid increasedgastrointestinal peristalsis and promoted gastric and bile secretion.Chlorogenic acid had a stimulant effect on the isolated rat uterus.

Di Gu Pi Cortex Lycii Radicis (Wolfberry Bark) Chemistry

Di Gu Pi contains 5α-stigmastan-3 6-dione, Betaine, Cinnamic acid,Kukoamine A, Linoleic acid, Lyciumamide, Melissic acid, Sugiol, andβ-sitosterol

Pharmacology Antipyretic Effect of Di Gu Pi

The aqueous or alcoholic extract of the herb, given orally or byinjection, produced a significant antipyretic effect in rabbits withfever induced by pyrogen. Betaine was also active. A strong antipyreticeffect was also exhibited by the aqueous fraction of the alcoholicextract at doses ranging from 0.75 to 7.5 g/kg equivalent of the crudedrug. The precipitates of the extract from lead salt also showedcomparable antipyretic activity to synthetic antipyretic analgesics.

Hypoglycemic Effect of Di Gu Pi

Oral administration of the decoction of the herb decreased blood glucoselevel in rabbits by 14% in average; this action lasted for 7-8 h. Thepeak action was observed 3 to 4 h after administration. It was also lesseffective when give subcutaneously. Subcutaneous dose of 6 g/kg of theextract elicited a mean reduction of 14% of the blood glucose of rabbitsafter 1 h.

Hypotensive and Anticholesterolemic Effect of Di Gu Pi

The decoction, macerate, tincture and injection solution of the herbproduced a significant hypotensive effect in anesthetized dogs, cats,rabbits by intravenous or intramuscular administration and inanaesthetized rats by oral administration. Repeated intravenousadministration at lower doses did not induce rapid tolerance.Intravenous injection of 0.375 g/kg of the injection solution resultedin sudden drop of blood pressure and death of anesthetized dogs.Bradycardia, prolongation of PR interval and depressed T wave in the ECGwere observed. Kukoamine A induced hypotension in rats when givenintravenously at a dose of 5 mg/kg.

Daily oral administration of 10 g/kg of the extract of the herb for 3weeks decreased the serum cholesterol in rabbits by 36.9% with littleeffect on triglyceride.

Antimicrobial Activity of Di Gu Pi

In the sensitivity test using the paper disc method, the decoction ofthe herb strongly inhibited Bacillus typhosus, Salmonella paratyphi A,and Shigella flexneri but was inactive against Staphylococcus aureus. Itwas a weak bacteriostatic against Mycobacterium tuberculosis. In theprimary monolayer tissue culture of the embryonic renal cells, thedecoction prevented the pathogenic changes in the cells due to Asianinfluenza virus A JK strain.

Effect on the Uterus of Di Gu Pi

The 100% injection solution of the bark showed stimulation effects onnormal rat uterus and isolated mouse uterus. The activity of 1 ml of thesolution was comparable to that of 0.054 unit of pituitrin.

Xian He Cao Herba Agrimoniae (Hairyvein Agrimonia Herb) Chemistry

Xian He Cao contains Agrimols A, B, C, D and E, Agrimoniin,

Agrimonolide, Agrimorphol, Apigenin-7-glucoside, Caffeic acid, Ellagicacid, Gallic acid, Luteolin-7-glucoside, Pendinculagin, Potentillin, andQuercetin

Pharmarcology Teniacidal Effect of Xian He Cao

The winter sprout of the herb is used in folk medicine to expel teniaand the active principle was found to be agrimophol. Agrimophol actsdirectly on the parasite. It inhibits the glycogenolysis, aerobic andanaerobic metabolism in the parasite.

The herb and agrimophol are also lethal to some other parasites, such asTrichomonas vaginalis, blood fluke and roundworm.

Antibacterial Activities of Xian He Cao

Six compounds, luteolin-7-glucoside, apigenin-7glucoside. Quercetin,ellagic acid, caffeic acid and gallic acid, isolated from the herb wereactive against bacillary dysentery. Combination use ofluteolin-7-glucoside and ellagic acid, apigenin-7-glucoside andapigenin-7-glucoside was more effective than the respective individualcompounds.

Antitumor Effect of Xian He Cao

Agrimoniin had antitumour activity when given as a pre- orposttreatment. A single dose of 10-30 mg/kg prolonged the life span ofmice bearing MM₂ tumors or yielded cures when given intravenously ororally prior to or after tumor inoculation. Agrimoniin also inhibitedthe growth of MH-134 and Meth-A solid tumors in mice. It was stronglycytotoxic to MM₂ cells in vitro, but the activity was almost completelyabolished by the addition of fetal calf serum to the culture.Intraperitoneal administration of agrimoniin increased the number ofperipheral white blood cells and the proportion of monocytes. Theantitumor activity of agrimoniin appeared to be due to its enhancementof the immune response.

Cardiovascular Effect of Xian He Cao

Intravenous administration of the alcoholic extract of the herbincreased blood pressure and stimulated respiration in anesthetizedrabbits and dogs, but the alcohol-soluble fraction of the aqueousextract lowered blood pressure in rabbits. Perfused into the bloodvessels of rabbit ear and frog hind limb, it caused vasoconstriction atlow concentrations and vasodilation at high concentrations. The extractand agrimoniin increased the heart rate and cardiac contractility offrogs and toads. On the other hand, the alcohol-soluble fraction of theaqueous extract inhibited the isolated frog heart.

Chun Gen Pi Cortex Ailanthi (Tree-of-Heaven Bark) Chemistry

Chun Gen Pi contains 1-(1′,2′-dihydroxyethyl)-4-methoxy-β-caboline,1-(2′-hydroxyethyl)-4-methoxy-β-carboline,1-(2-hydroxy-1-methoxy)-ethyl-4-methoxy-β-carboline,13(21)-dehydro-glaucarubinone, 13(21)-dehydroglaucarubolone,1-acetyl-4-methoxy-β-carboline, 1-carbamoyl-β-carboline,1-carbomethoxy-β-carboine, 1-hydroxycanthin-6-one,1-methoxycanthin-6-one, 1-methoxy-canthin-6-one-3-oxide,5-hydroxymethylcanthin-6-one, 6-methoxy-β-carboline-1-carboxylic methylester, Ailanthone, Ailantinols A and B, Amarolide, Amarolide 11-acetate,Canthin-6-one, Canthin-6-one-3-oxide, Chaparrinone, Chaparrolide,Glaucarubinone Quassinoids Δ¹³⁽¹⁸⁾-dehydro-glaucarubinone,Δ¹³⁽¹⁸⁾-dehydroglaucarubolone, Shinjudilactone, Shinjulactones A-N, andβ-carboline-1-propionic acid.

Pharmacology

Glaucarubinone and ailanthone showed amebicidal activity in vitroagainst the parasite Entamoeba histolytica. Some quassinoids markedlyinhibited the growth of chloroquine-resistant Plamodium falciparum.Glaucarubinone produced complete inhibition at 0.0006 μg/ml. Ailanthonealso showed potent antiulcer activity.

FIG. 19 illustrates the effect on live cells of three combinations ofherbs each in three concentrations 1:40, 1:20 and 1:10. C1 includesSheng Di Huang, C2 includes Jin Yin Hua, Da Huang, Mu Dan Pi and Di GuPi, and C3 includes Xian He Cao and Chun Gen Pi. FIG. 19 illustratesthat each of these three example combinations provides a reduction ofthe live cell numbers particularly in higher concentrations, while C1and C2 appear to be more effective than C3. Each of C1 and C2 includesone or two of the three herbs Sheng Di Huang, Jin Yin Hua and Da Huang,while C3 does not. In addition, the combination of four herbs in C2appears to be more effective than the single herb in C1.

FIGS. 20-23 illustrate plots for each of the eighteen herbs of growth asa percentage of control versus dilution factor. FIGS. 16-18 illustratethat the three herbs Sheng Di Huang, Da Huang and Jin Yin Hua are mosteffective, while the four herbs Mu Dan Pi, Di Gu Pi, Xian He Cao andChun Gen Pi are effective, and the eleven herbs Zi Cao, Xuan Shen, ShiGao, Bai Shao, Chi Shao, Hong Hua, Da Qing Ye, Qing Dai, Bai Zhu, ShiWei and Rou Gui are somewhat less effective.

FIG. 24 illustrates the effect on live cells of five combinations ofherbs each in two concentrations 1:40 and 1:80. Cal includes acombination of all eighteen herbs, C2 includes a combination of Jin YinHua, Da Huang, Mu Dan Pi and Di Gu Pi, C3 includes a combination of DaHuang, Sheng Di Huang and Jin Yin Hua, C4 and C5 include combinations ofthe other eleven herbs (Zi Cao, Xuan Shen, Shi Gao, Bai Shao, Chi Shao,Hong Hua, Da Qing Ye, Qing Dai, Bai Zhu, Shi Wei and Rou Gui) of theeighteen herbs, i.e., not in combination with any of the seven herbs(Sheng Di Huang, Da Huang, Jin Yin Hua, Mu Dan Pi, Di Gu Pi, Xian He Caoand Chun Gen Pi). Specifically, C4 includes Shi Gao, Shi Wei, Bai Zhu,Rou Gui, Hong Hua and C5 includes Zi Cao, Xuan Shen, Chi Shao, Bai Shao,Da Qin Ye, and Qing Dai. FIG. 24 illustrates that combinations of alleighteen herbs, as well as combinations of the three herbs (Sheng DiHuang, Da Huang and Jin Yin Hua) are most effective, while thecombination C2 was less effective, and the combinations C4 and C5 werestill less effective.

Herbal Ingredients Aloe Emodin

Aloe emodin has a molecular weight around 270.24 g/mol. As to itsanti-cancer activity, aloe emodin exhibits cytotoxicity in SCC oftongue, cervix cancer cells; and apoptoticity through MAPK-JNK cascadein hepatoma cells. Aloe emodin also tends to induce P53 and apoptosis.In addition, the cytotoxicity of aloe emodin induces effects inmelanoma, and gastric carcinoma. As to its anti-inflammatory activity,aloe emodin exhibits anti TNF and anti virality in envelopedviruses—HSV, PSV, VSV, and INF. Aloe emodin also decreases COX2 and INOSexpression in inflammation, and increases IFN in JEV and EV71. Aloeemodin may also be ingested for its properties as a laxative. Use ofaloe emodin can induce nausia, and intestinal contraction causingabdominal pain. Long use of anthraquinones can lead to kidney and liverdamage. The half-life of aloe emodin is about 78 min.

Chrysophanol

Chrysophanol has a molecular weight around 254.24 g/mol. As to itsanti-cancer activity, chrysophanol exhibits necrosis in hepatic cancercells, including ATP change and ROS cascade. Chrysophanic acid inhibitsEGFR in colon cancer cells. As to its anti-inflammatory activity,chrysophanol can serve as an antiseptic, bactericide, candidicide,and/or cathartic. In addition, chrysophanol can be used as an anti-staphaurus and an anti-bacilus subtilis. Chrysophanol can be used to suppressthe activation of NF-kB. and caspase-1 in LPS-stimulated macrophages.Chrysophanol is also an anti polio virus compound. Chrysophanol an beused as a hemostat and as a pesticide, and can be used in the treatmentof menorrhagia, including bleeding following abortion, epistaxis,functional uterine bleeding and thrombocytopenia. Chrysophanol acts as apurgative, and can be used to converts aloe emodin through P450 in theliver. Emodin and chrysophanol may be ingested in combination as an anticancer treatment agent. Chrysophanol has a half life of about 2.75hours.

Emodin

Also known as Rheum emodi, emodin has a molecular weight around 270.24g/mol. As to its anti-cancer activities, emodin exhibits proapoptoticity in prostate cancer cells through P53, and P21. Emodinincreases ROS, and improves chemotherapy effect in prostate cells whichare drug resistant. Emodin exhibits pro apoptoticity through inhibitionof IL6 in myltiple myaloma. Emodin exhibits anti-matastaticity0 throughintegrins effect. Emodin decreases HER2 in breast cancer and improveschemotherapoitic effect. Emodin induces cytotoxis in tongue carcinoma,and inhibits NFkB and other pro inflammatory cytokines. As to itsanti-inflammatory activities, emodin may be used as an anti ulcer agentthrough Hpylori destruction and change in gastric fluid. Emodin inducesa stabilizing effect on atherosclerotic plaque in vessels, and improvesinsulin and glucose changes in type 2 diabetes. Emodin promotes antiplasmodium against malaria, and may be used as an immunosuppressive,pesticide, purgative, spasmolytic, styptic, vasoelaxant, and/orviricide. Emodin has a half-life of approximately 227 min and convertsto two active metabolites through P450 in the liver.

Rhein

Also know as cassic acid, Rhein has a molecular weight around 284.22g/mol. As to its anti-cancer activity, rhein acts as an antiproliferative in hepatic carcinoma, breast cancer, SCC of lungs, andcervical cancer. Rhein improves taxol effect in breast cancer, andinhibits nasopharengeal carcinoma and EGFR. Rhein serves as a sinergistwith mitomycin. Rhein exhibits cytotoxicity in tongue carcinoma. Rheinmay be used for anti angiogenesis. As to its anti-inflammatory activity,rhein exhibits anti fibroticity, and promotes anti proliferation ofhepatic cells through inhibition of TGFb1. Rhein may be used as an antioxidant. Rhein decreases IL1B, and IL18 proinflammatory cytokines. Rheinis also anti bacterial, and may be used against staph. Aurus. Rhein alsoincreases sensitivity to ADH (drug). Rhein has a half-life of about 205min (approx). Rhein is hydrophobic, and may be combined with lysin inorder to be hydrophilic, as rhein lysinate.

Chrysophanein

Chrysophanein exhibits significant in-vitro cytotoxic activity in cancercell lines

Rhapontin

Rhapontin has a molecular weight of around 420.41 g/mol. As to itsanti-cancer activities, rhapontin induces apoptosis and suppresses KATOIII cell-growth in stomach cancer. Rhapontin also provides protectiveeffects on LDL and erythrocytes against oxidative damage. Rhapontin hasa half life of about 23.5 minutes.

Stilbene Piceid

Stilbene piceid has a molecular weight around 390.2 g/mol. As to itsanti-cancer activities, stilbene piceid inhibits DNA synthesis in LLCcells. Stilbene piceid also has an inhibitory effect on lipoxygenase.Stilbene also exhibits antioxidant activity and inhibitsalpha-glucosidase. Stilbene piceid also inhibits lipid peroxidationinduced by ADP and NADPH in liver microsomes. Stilbene acts directly onsmooth muscle to promote pulmonary artery relaxation.

Tannin

Tannin has a molecular weight around 500-3000 g/mol. As to itsanti-cancer activity, tannin suppresses the growth of MCF-7 breastcancer cells. Geraniin, a form of tannin separated from geranium, causescell death through induction of apoptosis. Tannin exhibits differentantiproliferative effects against cervical and colon cancer cells grownin vitro. In pomegranate, tannin modulates inflammatory cell signalingin colon cancer cells. Tannin promotes apoptosis through induction ofp53 non-small cell lung cancer cells. As to its anti-inflammatoryactivity, tannin in tomato suppresses COX-2 expression. Tannin can beused as an in vitro antioxidant and/or antiplatelet and also as ananti-inflammatory due to its free radical scavenging effects. Tannin maybe used for its antiviral, antibacterial and/or antiparasitic effects.Tannin can be used in the treatment of HFE hereditary hemochromatosis.Tannin is capable of reversing 6-hydroxydopamine induced toxicity.Tannin has a dental use, as well, as tannin-fluoride preparation reducesgingival inflammation around abutment teeth. A large intake of tanninsmay cause bowel irritation, kidney irritation, liver damage, irritationof the stomach and/or gastrointestinal pain. A correlation has been madebetween esophogeal or nasal cancer in humans and regular consumption ofcertain herbs with high tannin concentrations. Tannins inhibit theabsorption of minerals such as iron which may, if prolonged, lead toanemia. Tannins are present in soil, plants, water, tea, wine, andfruit. Tannin has a half life of about 3.15 hours.

Carvacrol

Carvacrol, also known as cymophenol, has a molecular weight around150.217 g/mol. As to its anti-cancer activities, carvacrol promotesanti-tumor effects on human metastatic breast cancer cells, includingMDA-MB 231. Carvacrol is a potent inhibitor of cell growth inHumanNon-Small Cell Lung cancer. Carvacrol also inhibits growth of myoblastcells even after activation of mutated N-ras oncogene. As to itsanti-inflammatory activities, carvacrol activates PPAR and suppressesCOX-2 inflammation. Carvacrol may be used for its antiproliferative andantiplatelet properties. Carvacrol is present in the essential oil ofOriganum vulgare, oil of thyme, oil obtained from pepperwort, and wildbergamot. Carvacrol inhibits the growth of several bacteria strains,e.g. Escherichia coli and Bacillus cereus, and in pseudomonasaeruginosa, carvacrol disrupts the bacteria membrane. Carvacrol may beused for its antioxidant activity. Carvacrol has a half life of about1.29 hours.

Eugenol

Eugenol has a molecular weight of about 164.2 g/mol. Eugenol inducesapoptosis in human colon cancer cells, and inhibits invasion andangiogenesis of gastric carcinogenesis induced by MNNG. Eugenol in honeysignificantly inhibits the growth of Ehrlich ascites carcinoma.Eugenol-related biphenyl (S)-6,6′-dibromo-dehydrodieugenol elicitsspecific antiproliferative activity on neuroectodermal tumour cellspartially triggering apoptosis. Eugenol causes melanoma growthsuppression through inhibition of E2F1 transcriptional activity. Eugenolmay also be used as an antiseptic. Eugenol also inhibits plateletaggregation induced by agonists, including collagen, ADP and calciumionophore. Eugenol may be extracted from certain essential oilsespecially from clove oil, nutmeg, cinnamon, basil and bay leaf. Eugenolis also used in perfumeries, flavorings, essential oils and in medicineas a local antiseptic and anesthetic. Eugenol may be used for itsantioxidative properties. Eugenol exhibits hepatotoxicity. An overdoseof eugenol may induce convulsions, diarrhea, nausea, unconsciousness,dizziness, and/or rapid heartbeat. Eugenol can be allergenic. Eugenolmay express carcinogenicity through oxidative DNA damage by itsmetabolite. Eugenol has a half-life of 1.975 hours, and under certainconditions, may have a half life up to 4 hours or even 18 hours.

Geraniol

Geraniol has a molecular weight of about 154.25 g/mol. As to itsanti-cancer activities, Geraniol promotes an antiproliferative mechanismin human pancreatic adenocarcinoma cells. Geraniol also promotesanti-proliferative and cell cycle regulatory effects in human breastcancer cells. Geraniol can cause a 2-fold reduction of thymidylatesynthase and thymidine kinase expression in colon cancer cells.Geraniol, as a component of plant essential oils, sensitizes human coloncancer cells to 5-fluorouracil treatment. Geraniol suppresses pancreatictumor growth without significantly affecting blood cholesterol levels.As to its anti-inflammatory activities, Geraniol diminishes the levelsof inflammatory markers induced by pamidronate stimuli in vitro and invivo. Geraniol also promotes inhibitory effects on nitric oxide andprostaglandin E₂ production in macrophages. Geraniol is a component ofrose oil, palmarosa oil, and citronella oil, and small quantities ofgeraniol are in geranium and lemon, has a rose-like odor and is commonlyused in perfumes. Geraniol can be used as effective plant-based mosquitorepellent. Geraniol is found in cigarettes. As to biologic use,ion-exchange iontophoresis combined with geraniol is a highly effectivetransdermal delivery system. Geraniol suppresses Candida cell growth inthe vagina and its local inflammation when combined with vaginalwashing. Gernaiol is also allergenic. Geraniol has a half life of about0.713 hours.

Luteolin

Luteolin has a molecular weight of 286.24 g/mol. As to its anti-canceractivity, luteolin, particularly in combination with standard anticancerdrugs such as cisplatin, serves as a HDAC inhibitor, e.g., for thetreatment of lung cancer. Luteolin promotes synergistic/additive growthinhibitory effects and may be effective in chemoprevention treatment ofhead and neck and lung cancers. Luteolin induces G1 arrest in humannasopharyngeal carcinoma cells. Luteolin not only protects DNA fromoxidative damage, but also increases repair activity in Caco-2 cells. Alow concentration of Luteolin has little toxic effect on cancer cells,but such low concentrations can sensitize chemotherapeutic drugs invarious cancer cell lines. Luteolin selectively inhibitschymotrypsin-like and trypsin-like proteasome catalytic activities intumor cells. Luteolin inhibits invasion of prostate cancer PC3 cellsthrough E-cadherin.

Luteolin is a PDE4 inhibitor and a general phosphodiesterase inhibitor,and an Interleukin 6 inhibitor. Luteolin inhibits inflammatory responseand improves insulin sensitivity in the endothelium. Luteolin preventsLPS-induced TNF-α expression in cardiac myocytes through inhibitingNF-κB signaling pathway. Luteolin inhibits myelin basic protein-inducedhuman mast cell activation and mast cell-dependent stimulation of JurkatT cells. Luteolin inhibits cyclooxygenase-2 expression and scavengesreactive oxygen species.

Luteolin is found in leaves, but it is also seen in celery, thyme,dandelion, rinds, barks, clover blossom and ragweed pollen. Luteolin isuseful in the prevention and treatment of skin photoaging. Luteolininhibits microglia and alters hippocampal-dependent spatial workingmemory. Luteolin enhances insulin sensitivity via activation of PPARγtranscriptional activity in adipocytes. Luteolin can induce nausea,vomiting and gastric hypersecretion. Luteolin has a half life of about1.2 hours.

Saponins

As to the anti-cancer activities of saponins with oleanolic acid,achyranthoside H methyl ester, a novel oleanolic acid saponin derivativefrom Achyranthes fauriei roots, induces apoptosis in human breast cancerMCF-7 and MDA-MB-453 cells via a caspase activation pathway. Saponionwith oleanolic acid exhibit insecticidal activity against the Mexicanbean beetle larvae (Epilachna varivestis).

Vanillic Acid

Vanillic acid has a molecular weight of 168.14672 g/mol. Vanillic acidsuppresses metastatic potential of human cancer cells through PI3Kinhibition and decreases angiogenesis in vivo. Vanillic acid enhancesthe activity of human lymphocyte proliferation and secretion ofIFN-gamma. Vanillic acid has a beneficial effect on DSS-inducedulcerative colitis, thereby manifesting its usefulness in the regulationof chronic intestinal inflammation. Phenolic compounds in mushroomLentinula edodes (shiitake) are hepatoprotective through theirsuppression of immune-mediated liver inflammation. Vanillic acid isfound in the root of Angelica sinensis, and in olive oil. Vanillic acidpromotes reduced cellular tyrosinase activity, DOPA oxidase and melanincontents, as well as down-regulated expressions of melanocortin-1receptor (MC1R), microphthalmia-associated transcription factor (MITF),tyrosinase, and tyrosinase-related proteins 2 (TRP-2) and TRP-1.Vanillic acid contributes to the prevention of the development ofdiabetic neuropathy by blocking the methylglyoxal-mediated intracellularglycation system. There exist an oxidized form of vanillin. Vanillicacis has a half life of about 10.552 hours.

α-Terpineol

The anti-cancer activities of α-terpineol, or alpha-terpineol, arepartly mediated through the suppression of NF-kappaB activation.α-terpineol exhibits antiproliferative effects on erythroleukemic K562cells. α-terpineol inhibits gene expression of the IL-6 receptor.α-terpineol suppresses fMLP-, LPS- and PMA-stimulated superoxideproduction. α-terpineol is found in cajuput oil, pine oil, andpetitgrain oil, and is a common ingredient in perfumes, cosmetics, andflavors and tea. α-terpineol demonstrates different degrees of growthinhibition against 15 different genera of oral bacteria. α-terpineol cancause postural hypotension in pine oil, and can cause eye irritation.There are three isomers, alpha-, beta-, and gamma-terpineol.Alpha-terpineol has a half life of about 1.245 hours.

Aucubin Aucubin has a Molecular Weight of 346.32978 g/mol

1. Antiproliferative activity is through cell cycle arrest and apoptosisin human non-small cell lung cancer A549 cells.

2. research—DNA damage induced by topoisomerase I poisoning as one ofthe possible mechanisms by whichaucubin have shown antitumoral activity.

3. research—cytotoxic activity against MCF7-breast adenocarcinoma,HeLa-cervix adenocarcinoma, A431-skin carcinoma of epithelial origin.

4. can obstruct H(2)O(2)-induced apoptosis by regulating of theexpression of Bcl-2 and Bax, as well as suppression of caspases cascadeactivation.

Aucubin enhance the activity of human lymphocyte proliferation andsecretion of IFN-gamma. Aucubin is found in the leaves of Aucubajaponica (Cornaceae), Eucommia ulmoides (Eucommiaceae), and Plantagoasiatic (Plantaginaceae). Aucubin protects against liver damage inducedby carbon tetrachloride or alpha-amanitin, particularly when dosedintra-peritoneally. Aucubin provides neuroprotection in primary diabeticencephalopathy. Aucubin treatment can lower blood glucose. Aucubin canproduce an increase in the level of lipid peroxidation and a decrease inactivities of antioxidant enzymes in liver and kidneys. Aucubin has ahalf life of about 42.5 minutes.

Digoxin

Digoxin, also known as digitalis, has a molecular weight of 780.938g/mol. Digoxin treatment can inhibit HIF-1alpha synthesis and blocktumor growth. Digoxin induces apoptosis in a human acute T-celllymphoblastic leukemia cell line. Digoxin can serve as a specificneuroblastoma growth inhibitor and an unspecific inhibitor ofangiogenesis.

Digoxin is widely used in the treatment of various heart conditions,namely atrial fibrillation, atrial flutter and sometimes heart failurethat generally cannot be controlled by other medication. Digoxinincreases myocardial contractility, such that the heart rate isdecreased, while blood pressure increases as stroke volume is increased,leading to increased tissue perfusion. Digoxin improves myocardialefficiency due to improved hemodynamics, and improves the ventricularfunction curve. Digoxin affects the kidney by increased renal blood flowand increased GFR. A mild diuretic effect is seen typically only inheart failure. Digoxin may cause AV junctional rhythm and ectopic beats(bigeminy) resulting in ventricular tachycardia and fibrillation.

Digoxin can induce loss of appetite, nausea, vomiting and diarrhea asthe gastrointestinal motility increases. Other common effects of Digoxinare blurred vision, visual disturbances (yellow-green halos and problemswith color perception), confusion, drowsiness, dizziness, insomnia,nightmares, agitation, and depression, as well as a higher acute senseof sensual activities. Less frequent adverse effects of digoxin(0.1%-1%) include: acute psychosis, delirium, amnesia, convulsions,shortened QRS complex, atrial or ventricular extrasystoles, paroxysmalatrial tachycardia with AV block, ventricular tachycardia orfibrillation, and heart block. Dangerous interactions can occur betweendigoxin and verapamil, amiodarone, erythromycin, and epinephrine. Theefficacy of chemotherapeutic agent substrates of Pgp may be stronglyreduced in patients taking digoxin. Digoxin treatment increases the riskof invasive breast cancer among postmenopausal women. Digoxin has ahalf-life around 36 hours.

Isoacetoside

Isoacetoside, as an extract from Tecoma stans, exhibits a cytotoxiceffect on human hepatocarcinoma cells (Hep-G2). Isoacetoside has a halflife of about 3.7-6.4 hours.

β-Sitosterol

β-sitosterol, or beta-sitosterol, has a molecular weight around 414.71g/mol. β-sitosterol may be used to treat prostatic carcinoma and breastcancer. β-sitosterol may have chemopreventive potential by virtue of itsradical quenching ability in vitro, with minimal toxicity to normalcells. β-sitosterol also attenuates beta-catenin and PCNA expression,making it a potential anticancer drug for colon carcinogenesis.β-sitosterol significantly inhibits the growth, and induces theapoptosis, of SGC-7901 human stomach cancer cells in vitro. The decreaseof the bcl-2/bax ratio and DNA damage may produce apoptosis induced bybeta-sitosterol in SGC-7901 human stomach cancer cells. β-sitosterolenhances tamoxifen effectiveness on breast cancer cells by affectingceramide metabolism. β-sitosterol has a proapoptotic effect that ismediated through the activation of ERK and the block of the PI3K/Aktsignal pathway in MCA-102 cells. Therefore, beta-sitosterol has a strongpotential as a therapeutic agent for preventing cancers such asfibrosarcoma. Beta-sitosterol is an effective apoptosis-promoting agentand that incorporation of more phytosterols in the diet may serve apreventive measure for breast cancer. An anti-microtubule characteristicof beta-sitosterol may contribute to the proliferation inhibition ofSiHa cells in cervical cancer. β-sitosterol activates the sphingomyelincycle and induces apoptosis in LNCaP human prostate cancer cells.β-sitosterol promotes anti-asthmatic actions that may be mediated byinhibiting the cellular responses and subsequent release/synthesis ofTh2 cytokines. β-sitosterol may have therapeutic potential in allergicasthma.

β-sitosterol is found in Nigella sativa, pecans, Serenoa repens (sawpalmetto), avocados, Curcurbita pepo (pumpkin seed), Pygeum africanum,cashew fruit, rice bran, wheat germ, corn oils, soybeans, sea-buckthorn,wolfberries, and Wrightia tinctoria.

β-sitosterol reduces blood levels of cholesterol, and is sometimes usedin treating hypercholesterolemia. β-sitosterol may produce a positiveeffect on male hair loss in combination with Saw palmetto. β-sitosterolmay play a major role in herbal therapy, especially in the treatment ofbenign prostatic hyperplasia. Beta-sitosterol is a naturally occurringphytosterol that may be used to cure atherosclerosis, diabetes, cancer,and inflammation and is also an antioxidant. β-sitosterol has a halflife of about 0.966 hours.

By taking more than the recommended dose of β-sitosterol, people maysuffer from stomach upset, nausea, diarrhea, gas or constipation,impotence (also known as erectile dysfunction or ED), decreased sexdrive. Beta-Sitosterol should be avoided during pregnancy andbreast-feeding, since it is not proven to be benign with regard topotential effects on unborn and newborn children. β-Sitosterol is alsonot recommended for individuals with sitosterolemia, a rare inheritedfat storage disease, because people with this condition have too muchβ-sitosterol and related fats in their system, taking β-sitosterol willonly worsen this condition. High levels of β-sitosterol concentrationsin blood have been correlated with increased severity of heart diseasein men having previously suffered from heart attacks, and may causeallergy.

Half-lives have been indicated for certain molecules. The half-lives ofmolecules can vary from these, e.g. generally based on the herb growingand/or preparation conditions, how it is combined with other herbs ofmolecules in treatment, or based on patient characteristics andbehaviors such as eating and drinking and physical activity, or onpotency or other factors. Doses and dose periods may be determined basedin part on the half lives. Typically, however, doses and dose periodswill be determined based on characteristics of the patient, thepatient's condition and the patient's history, as well as on theexpertise and experience of the attending physician.

The treatment method may include periodic shampooing with a formulaincluding one or more of the herbs described herein in combination withapproximately 5 ug/ml or 10 ug/ml or more of emodin, alone or incombination with respectively 0.05 ug/ml or 0.10 ug/ml or more ofdigoxin. Other combinations may be used in the treatment, includingcombining 5 ug/ml or more of emodin alone or with at least approximately0.10 ug/ml digoxin, or at least approximately 10 ug/ml emodin alone orwith at least approximately 0.10 ug/ml digoxin, or more than 5 ug/ml ofemodin alone or with at least approximately 0.05 ug/ml digoxin, or atleast approximately 10 ug/ml emodin alone or with at least approximately0.05 ug/ml digoxin. Other combinations may be used and prescribed byphysicians depending on factors such variances in weight, age, gender,family or patient history, or other characteristics specific topatients.

The shampoo regimen may include once or twice daily shampoo treatments,or two or more weekly doses weekly or otherwise. Doses may be taken morethan once or twice a day, while the amounts of each dose would bedetermined according to the periodicity of the treatments.

Methods of preparing shampoo as a medicinal treatment are also provided,including preparing a shampoo formula that includes Sheng Di Huang, DaHuang or Jin Yin Hua, or combination thereof, alone or in combinationwith another herb or molecule described herein or as understood by thoseskilled in the art.

A hair or scalp shampoo is also provided, including one or more of ShengDi Huang, Da Huang or Jin Yin Hua and/another herb or molecule describedherein or as understood by those skilled in the art.

FIG. 19 illustrates the effect of proliferation of digoxin, emodin andtheir combination on MDA 435 are cell lines of Melanoma and BreastCancer. In this case, concentration of 1/100 had no effect (same asControl) 1/3 had good effect and 1/10 killed all cells. Normal cellswere affected 50-90% less than the rate of pathological cells.Treatments described herein may be prepared for topical use fortreatment of melanoma, BCC (basal cyr carcinoma) and inflammatory skindiseases like Eczema. For example, combinations of herbs and/or herbalextracts as described herein may be prepared as a cream to apply ontothe skin or as a shampoo, conditioner or other hair and scalp cleaningor rinsing agent. Herb or herb extract combinations described hereinalso may be injected to infected areas of the scalp or other skin regionof a patient using a syringe, or they may be taken orally orintravenously. An example method for preparation of an external cream inaccordance with certain embodiments is provided below.

Preparation of External Cream/Ointment

First, one of more of the herbs may be cooked, for example, as describedelsewhere herein or as may be understood or determined by those skilledin the art. Da Huang may be cooked for 5 or 10 minutes or more. Apurgative property of Da Huang may be reduced by cooking for 15 minutesor more. The herbs may be filtered through a 0.2 micron filter. Theherbs may be centrifuged. A cream is then prepared that may be somewhatmore or less than half herbs and half cream, e.g., a 30% liquid of herbsin 1:1 ratio and 70% cream may be used. The herbs can also be preparedas a tincture, e.g., soaking the herbs in alcohol for a period of timesuch as 2 weeks in a ratio of 1:3, for example. This herbal liquid canthen be mixed with the cream in the same way as described above.

Shampoo treatments described herein may also be effective againstimmunodeficiency diseases such as HIV and AIDS, as well as otherconditions affecting the immune system and affecting the hair or scalp.

EXAMPLES

The embodiments are directed to advantageous medicines prepared as ashampoo or other hair or scalp eczema treatment and methods of treatmentfor eczema and preparation of eczema medicines and treatments foreczema, and/or other conditions such as psoriasis, melanoma or anotherskin condition formulated as a shampoo, conditioner, ointment, spray, orother topical scalp or hair treatment including an herbal combinationwith certain herbs such as Da Huang and Sheng Di Huang, or Da Huang,Sheng Di Huang and Jin Yin Hua, and/or Mu Dan Pi, Di Gu Pi, Xian He Caoand/or Chun Gen Pi, and/or any one or more of the eleven additionalherbs including Zi Cao, or radix arnebiae (arnebia root) or radixlithospermi (gromwell root), Xuan Shen, or radix scrophulariae (figwortroot), Shi Gao or gypsum fibrosum (gypsum), Bai Shao, or radix paeoniaealba (white peony root), Chi Shao or radix paeoniae rubra (red peonyroot), Hong Hua or flos carthami (safflower), Da Qing Ye or foliumisatidis (woad leaf), Qing Dai or indigo naturalis (natural indigo), BaiZhu or rhizoma atractylodis macrocephalae (largehead atractylodesrhizome), Shi Wei or folium pyrrosiae (shearer's pyrrosia leaf), and/orRou Gui or cortex cinnamomi (cinnamomum bark), and/or combinations ofcertain herbal ingredients, and/or one or more herbal ingredientsdescribed herein including Emodin, Rhein, and/or Rhapontin of Da Huang,Carvacrol, Vanillic acid, and/or Sitosterol of Jin Yin Hua, and/orAucubin, Digoxin, and/or beta-sitosterol of Sheng Di Huang and/or otheringredients present within the two, the three, the seven and/or even theeighteen herbs, may be included in a shampoo treatment in accordancewith certain embodiments.

In one embodiment, a shampoo treatment may include applying a dose, suchas in a process of shampooing the hair and scalp, of active herbalingredients, extracts or molecules to the scalp and hair from an example16 fluid ounce formulation that includes between 13.3 grams 30 to 60grams of Sheng Di Huang, or 3.3 grams to 30 grams of Da Huang, or both,alone or in combination with one of more other herbs or molecules to beadministered daily or 2-3 times daily or up to 10× daily or less thandaily even as little once or twice a week. In other embodiments, theshampoo formulation further includes 3.3 grams to 30 grams of Jin YinHua.

In other embodiments, two of these three are included in the shampooregimen. In further embodiments, one, two, three or all four of theherbs Mu Dan Pi, Xian He Cao, Chun Gen Pi and Di Gu Pi is/are combinedwith one, two or all of the three herbs Sheng Di Huang, Da Huang and JinYin Hua within a 16 ounce example shampoo or hair lotion formulation,including 3.3 grams to 15 grams of any of the other four of the sevenherbs, e.g., 3.3 grams to 15 grams of Mu Dan Pi, 3.3-10 grams to 15grams of Xian He Cao, 3.3-10 grams to 15 grams of Chun Gen Pi, and/or3.3-5 grams to 15 grams of Di Gu Pi.

In one example, an example 16 fluid ounce shampoo formulation includes40 grams of Sheng Di Huang, 15 grams of Da Huang and 15 grams of Jin YinHua with multiple other ingredients described in examples herein to beadministered as a shampoo regimen. The combination of herbs may becooked once or twice, and the herb:shampoo/lotion ratio may be 1:20 or1:40 or otherwise. The dose may be 1-10 days or even multiple timesdaily including 2-6 times daily, and even as often as 10 times daily.

In another example, a shampoo regimen includes 5 grams of Mu Dan Pi, 20grams of Sheng Di Huang, 5 grams of Xian He Cao, 5 grams of Chun Gen Pi,5 grams of Di Gu Pi, 10 grams of Da Huang and 10 grams of Jin Yin Hua ina 16 fluid ounce shampoo or hair lotion formulation. The combination maybe cooked once or twice, and the herb:shampoo/lotion ratio may be 1:10,1:20, 1:40 or otherwise. The dose may be 1-10 days or even multipletimes daily including 2-6 times daily, and even as often as 10 timesdaily.

In another example, a shampoo regimen is supplemented by a low doseherbal formula including 3.3 grams of two or more of Da Huang, Sheng DiHuang and Jin Yin Hua, and/or one or more of the following seven herbs:3.3 grams of Da Huang, 13.3 grams of Sheng Di Huang, 3.3 grams of JinYin Hua, 3.3 grams of Mu Dan Pi, 5 grams of Di Gu Pi, 10 grams of XianHe Cao, and 10 grams of Chun Gen Pi in water or in a dry or a lipidizedformulation or otherwise configured for topical, oral, intravenous orsub-dermal injectable administration.

In another example, a shampoo regimen includes a high dose herbalformula including 30 grams or more of Da Huang, and/or with one or moreof the following seven herbs: 60 grams of Sheng Di Huang, 30 grams ofJin Yin Hua, 15 grams of Mu Dan Pi, 15 grams of Di Gu Pi, 15 grams ofXian He Cao, and 15 grams of Chun Gen Pi, in an example 16 fluid ounceformulation, including other shampoo ingredients as may be described inexample embodiments herein, or the herbal formula may be dissolved inwater or prepared in a dry or a lipidized formulation or otherwiseconfigured for topical, oral, intravenous or sub-dermal injectableadministration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a formula that includes the three herbs including3.3-20 grams of Da Huang, 10-60 grams of Sheng Di Huang, and 3.3-20grams of Jin Yin Hua together with multiple other ingredients in anexample 16 fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a formula including 3.3-20 grams of Da Huang and10-60 grams of Sheng Di Huang in a 16 fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a formula including 3.3-20 grams of Jin Yin Huaand 10-60 grams of Sheng Di Huang in a 16 fluid ounce bottle.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a formula including 3.3-20 grams of Da Huang and3.3-20 grams of Jin Yin Hua in a 16 fluid ounce bottle.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo formula including one active herb, e.g.,3.3-30 grams of Da Huang, in a 16 ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo formula including one active herb, e.g.,3.3-30 grams of Jin Yin Hua, in a 16 ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including oneactive herb, e.g., 10-90 grams of Sheng Di Huang, in a 16 fluid ounceformulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including threeactive herbs including 10-100 grams of Da Huang, 25-250 grams of ShengDi Huang, and 10-100 grams of Jin Yin Hua in a 16, 32 or 64 fluid ounceformulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including 10-100grams of Da Huang and 25-250 grams of Sheng Di Huang in a 16, 32 or 64fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including 10-100grams of Jin Yin Hua and 25-250 grams of Sheng Di Huang, in a 16, 32 or64 fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including 10-100grams of Da Huang and 10-100 grams of Jin Yin Hua in a 16, 32 or 64fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including oneherb, e.g., 10-100 grams of Da Huang, in a 16, 32 or 64 fluid ounceshampoo or hair lotion formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula including oneherb, e.g., 10-100 grams of Jin Yin Hua, in a 16, 32, or 64 fluid ounceshampoo or hair lotion formula.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion including 25-250 grams ofSheng Di Huang in a 16, 32 or 64 fluid ounce shampoo or hair lotionformulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion that includes 30-300grams of Da Huang, 60-600 grams of Sheng Di Huang, and 30-300 grams ofJin Yin Hua in a 32-640 fluid ounce shampoo or hair lotion formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes30-300 grams of Da Huang, and 60-600 grams of Sheng Di Huang in a 32-640fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formulation that includes30-300 grams of Da Huang and 30-300 grams of Jin Yin Hua in a 32-640fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes60-600 grams of Sheng Di Huang and 30-300 grams of Jin Yin Hua in a32-640 fluid ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes30-300 grams of Da Huang in a 32-640 ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes30-300 grams of Jin Yin Hua in a 32-640 ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes60-600 grams of Sheng Di Huang in a 32-640 ounce formulation.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes2-10 grams of Da Huang in a 10 fluid ounce shampoo or hair lotionformula, or the Da Huang may be formulated in dry or lipidized form fororal, topical, sub-dermal or IV administration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes5-25 grams of Sheng Di Huang in a 10 fluid ounce shampoo or hair lotionformula, or the Sheng Di Huang may be formulated in dry or lipidizedform for oral, topical, sub-dermal or IV administration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes2-10 grams of Jin Yin Hua in a 10 fluid ounce shampoo or hair lotionformula, or the Jin Yin Hua may be formulated in dry or lipidized formfor oral, topical, sub-dermal or IV administration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes10-20 grams of Da Huang, 20-40 grams of Sheng Di Huang, and 10-20 gramsof Jin Yin Hua in a 32-64 fluid ounce shampoo or hair lotionformulation, or the active herbal combination may be formulation in dryor lipidized form for oral, topical, sub-dermal or IV administration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes1.5-15 grams of Da Huang, 3.5-35 grams of Sheng Di Huang, and 1.5-15grams of Jin Yin Hua in about 300 grams of shampoo or hair lotionproduct, or the herbal combination may be formulated in dry or lipidizedform for oral, topical, sub-dermal or IV administration.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo formula that include 25-75 grams of DaHuang, 45-135 grams of Sheng Di Huang, and 25-75 grams of Jin Yin Hua inabout 250-2000 grams of shampoo product solution. In another example, ashampoo regimen includes periodic applications to the hair and scalp ofa shampoo formula that includes 3.3-10 grams of Da Huang, and 13.3-40grams of Sheng Di Huang in about 100-300 grams of shampoo productsolution.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo formula that includes 30-100 grams of DaHuang and 60-200 grams of Sheng Di Huang in about 600-6000 grams ofshampoo or hair lotion product solution.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formulation that includes10-50 grams of Da Huang and 30-150 grams of Sheng Di Huang in about400-2000 grams of shampoo or hair lotion product solution.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes20-50 grams of Da Huang and 40-100 grams of Sheng Di Huang in about600-5000 grams of shampoo or hair lotion product solution.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion product that includes15-100 grams of Da Huang and 45-300 grams of Sheng Di Huang in about600-4000 grams of shampoo or hair lotion product solution.

In another example, a shampoo regimen includes periodic applications tothe hair and scalp of a shampoo or hair lotion formula that includes25-75 grams of Da Huang and 50-150 grams of Sheng Di Huang in about 800grams of shampoo or hair lotion product solution.

Examples of low, medium and high concentration herbal shampoo and/orhair lotion products or oral, IV, dermal adhesive or sub-dermalformulations may include one or more of the herbs described herein, suchas any combination of the herbs contained in the seven herb and eighteenherb combinations described herein, that are administered in doses thatare applied to the hair and scalp as a shampoo or hair lotion and thatare the same as or similar to or commensurate with the low, medium andhigh dose example formulas for the one, two and three herb combinationsdescribed above, including corresponding percentage changes when four ormore herbs are included in the supplemental herbal formula that isadministered before during or after a shampoo dose or regimen. Manyother combinations may be used depending on characteristics of thepatient such as age and weight, the condition of the patient, and thepatient's history.

Doses in between the low dose and high dose examples for the formulasthat include one, two, or three or more herbs, or the seven herbformula, or the eighteen herb formula are also within the scope offurther examples with per herb doses and/or total herb doses that arewithin the ranges provided in the examples above. Under certainconditions, doses above the high dose formula or below the low doseformula may be used as determined by a physician using his or herexpertise and experience both generally in the field and with specificpatients. In addition, other combinations of two, three, four, five, ormore of these 18 herbs, and/or including one or more other herbs asunderstood by those skilled in the art, may be used in further examplesof formulas wherein the dose ranges described in the above examples orotherwise as determined by a physician may be used. Further herbs notdescribed herein may also be included in formulas including combinationsof the described herbs and/or molecules, molecular extracts or molecularcompounds described herein or understood by those skilled in the art.

In the examples, certain doses of herb combinations have been describedin certain amounts of shampoo or hair lotion product solution. Mixingthe herbs in water and then into one of the example shampoo formulaedescribed herein is an example of a way to prepare the herbal shampoo orhair lotion products. Another liquid such as DMSO or an oil may be usedinstead of or in addition to water. The herbs may be integrated with theother components of the shampoo formula in a variety of ways includingmixing with one of the components before integration with the rest ofthe shampoo. The herbs may be formed into powders or dissolvable solidsor the herbs may be dissolved into solution and poured or a syringe orfluid transport tubing may be used. Any of the herbal combinations maybe formed into a shampoo treatment or rinse, or as cream and rubbed ontothe skin or hair, or dissolved into a solution such that a syringe maybe used to inject a patient sub-dermally with an herbal combination.

In another example, the effect of CXCR4 antagonists on the survival ofNB4, HL60, Jurkat leukemic cells and HT29 colon cancer and prostatetumor cells was examined. Digoxin significantly inhibited the growth ofleukemic cells at concentrations between (0.05- to 1 microgram/ml).Emodin by itself inhibited the growth of leukemic cells only atconcentrations of more than 5 microgrm/ml. Combinations of digoxin atconcentrations of 0.1 microgram/ml and either 5 or 10 microgram/ml ofemodin increased significantly the tumor killing ability of bothcompounds. Digoxin, Emodin, and their combination also add partialhowever significant effect on HT29 tumor cells.

The effect of the proliferation of digoxin, emodin, and theircombination on the viability of cancer cell lines from different originwas studied. Harvested non adherent human hematopoietic cancer celllines NB4, HL60 and Jurkat, cells were seeded at 2×105 viable cells/1 mlper well into a 24-well plate in triplicates in a medium supplementedwith 10% FCS and incubated with different concentrations of digoxin,emodin, and their combination for 24 hours. Following the incubation,the cells were stained with propidium iodide (PI) (Sigma, St. Louis,Mo.) and percent of viable PI-negative cells in culture was determinedby FACScalibur analysis (Becton Dickinson Immunocytometry Systems),using CellQuest software. Adherent prostate cancer PC3 cells and coloncancer HT29 cells were seeded at 1×10^(0.5) viable cells/1 ml per wellinto a 24-well plate under conditions described above, and following a24-hour exposure to digoxin, emodin, and their combination the cellswere harvested, washed with PBS and stained with PI and counted asdescribed for hematopoietic cells.

The therapeutic index of digoxin is understood to be between 0.125 m″gto 0.5 m″g, while the LD50 values of emodin in mice are understood to be0.56 g/k″g.

When combining D and E in accordance with certain embodiments, and/orcombining digoxin or emodin, or both, with one or more of the herbs,there is a surprising and unexpected symbiotic effect.

Certain treatments may be prepared as a mixture of herbs that are knownto have separately digoxin “D” and emodin “E”. Aloe-emodin is a naturalanthraquinone compound that is present in some traditional medicinalplants such as Rhei Rhizoma and Rheum palmatum. Interestingly,aloe-emodin has been found to have lesser cytotoxicity towards normalhuman cells. In-vitro tests with synthesized D&E molecules have beenperformed on a cell-line. The in-vitro with the plants was similar tothe one with the isolated molecules.

Emodin may be extracted from traditional medicinal plants such as RheiRhizoma and Rheum Palmatum. In one embodiment, the source of Emodin usedis Da Huang—Chinese name, or Rhubarb Root—English name, or RheumPlamatum—Botanical name, or Radix Rhisoma Rhei—Pharmaceutical name.Emodin may be extracted from Rhubarb, Buckthorn and/or Japanese Knotweed(Fallopia Japonica). Aloe-emodin may be used which is a variety ofemodin found in Socotrine, Barbados, and Zanzibar aloes.

A medicinal cancer treatment using Emodin is prepared in certainembodiments by mixing the herb, Da Huang, in water at a ratio ofapproximately 10:1. The herb may be ground to a fine powder. The watermay be added to the fine powdered herb, and the pot covered. Afterboiling, the heat is lowered in certain embodiments to about 70 degreescentigrade. The aqueous mixture is cooked for another hour. The liquidis then strained into a container. In some cases, this may be donetwice. In the second cooking, the ratio may be reduced to 7.5:1. Thesecond cooking may take about 45 minutes including the boiling.

Single herbs or combinations of two or more herbs alone or with any oneor more of the described molecules may be prepared in a processinvolving the following or a subset or variation thereof: grinding theherbs to a fine texture in the mixer for around 2-3 min until it lookedfine powder; weighing the powder (e.g., 25 gm) and transferring to abeaker (e.g., 2000 ml); adding distilled water (RT) to powdered herbs ina ratio of 1:20 (gm of herbs:ml of water) and soaking the herbs for15-20 min; boiling the mixture to 85-9C0 C; cooling the temperature ofthe mixture down to 70-75° C. after removing it from the hot plate;covering the beaker properly with aluminum paper and cooking the mixtureat 70° C. on hot plate—the total time of cooking of the mixture may beapproximately 60 min which includes boiling, cooling it down andcooking; straining the mixture with the help of a manual strainer; afterfiltration, centrifuging the extract at 5000 rpm for 15 min andcollecting the supernatant; filter sterilizing the supernatant bypassing through 0.2 m syringe filter; storing the clear filtrate at 4°C.-subsequent dilutions may be prepared.

The source of Digoxin in certain embodiments is Sheng Di Huang-Chinesename, or Foxglove root-English name, or Radix Rhemania-Pharmaceuticalname. The preparation of the Digoxin may be the same as for the Emodin.In certain embodiments, the herbs from which the Emodin and Digoxin areextracted are cooked together. Digoxin may be extracted from DigitalisPurpurea or Purple Foxglove.

In certain embodiments, the prepared treatment is not pure. For example,certain treatments may involve a “vegetable soup” type regiment made ofmore than one and even two, three, seven or eighteen herbal ingredientsper specific examples provided herein, or any combinations of the herbsdescribed herein, or combinations of the herbs described herein withother herbs not mentioned herein. In certain embodiments, herbalingredients are mixed in solution and a patient may drink the liquid.While freeze-drying the herb in powder form may be possible, theabove-described process appears to be more effective.

Harvested non adherent human hematopoietic cancer cell lines NB4 (AML),HL60 and Jurkat were seeded at 2×10⁵ viable cells/1 ml per well into a24-well plate in triplicates in a medium supplemented with 10% FCS andincubated with different concentrations of digoxin, Emodin, and theircombination for 24 hours. Following the incubation, the cells werestained with propidium iodide (PI) (Sigma, St. Louis, Mo.) and percentof viable PI-negative cells in culture was determined by FACScaliburanalysis (Becton Dickinson Immunocytometry Systems), using CellQuestsoftware. Adherent prostate cancer PC3 cells and colon cancer HT29 cellswere seeded at 1×10⁵ viable cells/1 ml per well into a 24-well plateunder conditions described above, and following 24-hour exposure todigoxin, Emodin, and their combination, the cells were harvested, washedwith PBS and stained with PI and counted as described for hematopoieticcells.

A scalp, hair or other skin condition treatment formulated as a shampoo,conditioner, cream, ointment, spray or other topical scalp, hair or skintreatment including an herbal combination of Da Huang and Sheng DiHuang, or Da Huang, Sheng Di Huang and Jin Yin Hua, or othercombinations of the herbs and/or molecules described herein may also becombined with other treatments described herein and/or as may beunderstood by those skilled in the art and/or as may be described inliterature such as the following which are hereby incorporated byreference as disclosing alternative embodiments and compounds that maybe combined with Da Huang, Sheng Di Huang or Jin Yin Hua, orcombinations thereof, in a cocktail or otherwise effective shampootherapy:

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A treatment regimen for eczema, and/or another skin and/or scalpcondition such as dandruff, melanoma or another scalp condition includesin certain embodiments an herbal combination of Sheng Di Huang and DaHuang, otherwise referred to herein as the three herb combination or“2-HX.” Another treatment regimen for eczema, and/or another disordersuch as psoriasis, dandruff, melanoma or another scalp conditionincludes an herbal combination of Jin Yin Hua, Sheng Di Huang and DaHuang, otherwise referred to herein as the three herb combination or“3-HX.” Another treatment regimen of periodic doses of an advantageouscombination of emodin and digoxin is provided to treat eczema. Anothertreatment regimen for eczema includes a combination of emodin (E) anddigoxin (D) with Jin Yin Hua, Sheng Di Huang and Da Huang, otherwisereferred to herein as the three herb combination or “3-HX.”. Anothertreatment regimen for eczema includes a combination of emodin anddigoxin, or D&E, and Jin Yin Hua, Sheng Di Huang and Da Huang or 3-HX,with Mu Dan Pi, Di Gu Pi, Xian He Cao and Chun Gen Pi, which forms acombination of D&E and the seven herb combination referred to herein.

The herbal combination may be formulated as a shampoo, conditioner,cream, ointment, spray or other topical scalp or hair treatment. Theherbal combination and/or the treatment regimen may be administeredtopically, orally, subcutaneously or intravenously. The treatmentregimen may include both the herbal combination and another medicinaltreatment such as any of the other treatments described herein oranother known or experimental treatment. The herbal combination canserve to enhance the potency of another scalp treatment and/or to reducetoxic side effects and/or dosage of another scalp treatment.

Anti-psoriatic activity of 3-HX, and of D&E was determined in studiesusing 12-O-Tetradecanoylphorbol-13-Acetate (TPA) induced earinflammation in male C57BL/6 mice. All the animals were randomized basedupon the body weight and allotted to seven groups with 6 animals in eachgroup. Groups G1 and G2 were treated topically with test item 3HX at thedose levels of 1:4 and 1:8 dilutions respectively. Groups G3 and G4 weretreated orally with test item 3HX at the dose levels of 500 and 1000mg/kg respectively. Group G5 and G6 were treated topically and orallywith D and E respectively. Group G7 served as both TPA control (rightear) and solvent control (left ear) and was treated orally with Na-CMC.

Among the various experimental groups topical application of 3-HX (1:4)and D&E and oral treatment of low dose of 3-HX (500 mg/kg) and D & Eexhibited maximum reduction in ear thickness. Similar trend of testitems response was observed in TPA-induced ear thickness change. Topicalapplication of test items exerted optimum % inhibitory activity againstTPA induced ear inflammation in mice whereas oral treatment exhibitedmarginal anti-inflammatory activity. In the punch biopsy weightinvestigation maximum reduction was obtained in topically D & E and 3-HX(1:4) treated groups whereas marginal reduction was obtained on oraltreatment with test items. The epidermal ear thickness results obtainedfrom histopathological photographs were in accordance with punch biopsyweight results where topical application of test items was found toexert better effect in comparison with oral treatment. Among theevaluation of test items over inflammatory biomarkers viz., enzymemyeloperoxidase (MPO) and nitric oxide levels, topical application of D& E and 3-HX (1:4) resulted in marked suppression of enzyme MPO activitywhereas marginal inhibition of nitric oxide content generation wasobtained on oral administration of test items respectively. Thus, basedupon the present experiment findings it is suggested that topicalapplication of 3-HX at the ratio of 1:4 and D & E as well as oraltreatment of low dose of 3-HX (500 mg/kg) may act as promising drugcandidate for the treatment of eczema.

TEST ITEM INFORMATION TEST ITEM-1 Name of the test item/code: 3-HX LotNo.: NA Pack Size: NA Physical Description: NA Storage condition: RoomTemperature Test Item Analysis: Analysis for the identity and purity ofthe test item was not conducted as part of this study, and is theresponsibility of the sponsor TEST ITEM-2 Name of the test item/code:Digoxin (D) Lot No.: 051M1374V Pack Size: NA Physical Description: Whiteto off white powder Storage condition: At Room Temperature Test ItemAnalysis: Analysis for the identity and purity of the test item was notconducted as part of this study, and is the responsibility of theSponsor Precautions in handling: Avoid contact with skin and eyes TESTITEM-3 Name of the test item/code : Emodin (E) Lot No.: M3074 Pack Size:NA Physical Description: Orange powder Storage condition: Stored at +4°C. Test Item Analysis: Analysis for the identity and purity of the testitem was not conducted as part of this study, and is the responsibilityof the sponsor Precautions in handling: Avoid contact with skin and eyesVEHICLE CONTROL 2% DMSO in methanol TEST SYSTEM Species: Mus musculusStrain: C57BL/6 Source: Animal Facility, Dabur Research Foundation Sex:Male Age: 6-8 Weeks Body weight range : 16.47-36.51 g Acclimatization:12 Days Randomization: Animals were randomized based on their bodyweight Identification of animals: By cage labeling and tail markingTotal no of animals: 42 Total no of groups:  7 Total no of animals pergroup:  6 Total no of animals per cage:  6 ANIMAL HUSBANDRY CONDITIONSRoom temperature : 21.8 to 24.2° C. Relative humidity: 56 to 59%Light/dark cycle: 12-hourly Feed: Conventional feed purchased from aGolden feed, Mehrauli was provided ad libitum to the animals. Water:Filtered drinking water was provided ad libitum.

Justification for Selection of Test System

Male C57BL/6 mice were selected as the test system, as they werecommonly reported in literature to evaluate the effect of test item foranti-psoriatic potential on TPA induced ear inflammation model.

CHEMICALS Catalogue No. Lot No. Name of the Chemical or CAS No. Companyor Batch No. Phorbol 12-myristate 13-acetate P8139-1MG Sigma lifesciences, SLBC5412V (TPA) USA Dimethyl sulfoxide (DMSO) 67-68-5 MerckSpecialties Pvt 80291205001730 Ltd., Mumbai, India Methanol M0275 RANKEMFine R106M10 Chemicals Ltd. New Delhi, India Sodium dihydrogen phosphate17845 Merck Specialties Pvt DL1DR511556 2-hydrate cryst. Pure Ltd.,Mumbai, India Hexadecyltrimethyl-ammonium H5882 Sigma life sciences,120M0141V bromide USA o-Dianisidine Dihydrochloride 195136 MOBiomedicals LLC M1271A di-Sodium hydrogen phosphate 7558-79-4 MerckSpecialties Pvt MF7M571743 anhydrous Purified Ltd., Mumbai, IndiaHydrogen Peroxide Solution 7722-84-1 Fisher Scientific 1889 7202-5Orthophosphoric acid 7664-38-2 Fisher Scientific 1309 7100-5Sulphanilamide 63-74-1 Central Drug House 930308 Pvt Ltd. New DelhiGlycine 98% 56-40-6 Acros Organics, USA A013597501 Sodium chloride7647-14-5 Merck Specialties Pvt MJ0M602975 Ltd., Mumbai, IndiaHydrochloric acid min. 35% 7647-01-0 Merck Specialties Pvt HD7H570285 GRLtd., Mumbai, India

Method Principle

Excessive proliferation of keratinocytes is a characteristic of eczema,and this cell type is a well reported target of therapy for thisdisease. In the present study, chronic skin inflammation was induced byrepeated topical application of TPA, which is recognized by prolongedskin reaction & epidermal hyperplasia. In this model the potential ofthree test items—3-HX, Digoxin (D) and Emodin (E) were evaluated forreduction of epidermal hyperplasia reflected by change in ear thickness& keratinocyte proliferation in C57BL/6 mice.

Experimental Procedure

The study was conducted on healthy, adult, male C57BL/6 mice. All theanimals were acclimatized to laboratory condition prior to experimentinitiation.

5 mg/ml stock of TPA was prepared in DMSO and aliquot of the same wasdiluted to 1:50 with methanol to achieve final TPA concentration of 100μg/ml for topical application. For oral application of 3-HX, a stocksolution of 100 mg/ml was prepared in distilled water and was furtherdiluted to 50 mg/mL, in-order to administer the dose of 500 mg/kg and1000 mg/kg. For topical application, required amount of 3HX wasdissolved in DMSO to obtain the final dilution of 1:4 and 1:8. For oralapplication of test item D, 10 mg/ml was prepared in DMSO and wasfurther formulated with 0.25% Na-CMC to obtain final strength of 0.1mg/ml. For topical application, required amount of D was dissolved inDMSO and methanol and stored at −20° C. Similarly, for oral applicationof test item E, 10 mg/ml stock was prepared in 0.25% Na-CMC and 0.1%Tween 80. For topical application, required amount of E was dissolved inDMSO and diluted with methanol.

On Day 0, all the animals were randomized based on body weight andallotted to seven groups containing 6 animals per group. Groups G1 andG2 were treated topically with test item 3HX at the dose levels of 1:4and 1:8 dilutions respectively. Groups G3 and G4 were treated orallywith test item 3HX at the dose levels of 500 and 1000 mg/kgrespectively. Group G5 was treated topically with test item D and E, incombination, at the concentration of 1 μg and 100 μg respectively. GroupG6 was treated orally with test item D at 1 mg/kg. After 30 min. of Dadministration, test item E was dosed orally at 100 mg/kg. Group G7served as both TPA control (right ear) and solvent control (left ear)and was treated orally with Na-CMC.

20 μL of TPA solution containing 2 μg of TPA in vehicle (2% DMSO and 98%methanol) was applied topically on both ventral and dorsal side of theright ear of all the groups of animals on day 0, 2, 4, 7 and 9. However,the left ear of Group G7 animals was treated topically with 20 μL ofsolvent (2% Dimethylsulfoxide+98% methanol) on day 0, 2, 4, 7 and 9.

All the test item formulations was administered orally to Groups-G3, G4,and G6 at the dose volume of 10 ml/kg from day 0 to day 9. For topicalapplication, 20 μL of test items (3HX and D+E) was dissolvedappropriately in the TPA solvent i.e. methanol and applied daily fromDay 0 to Day 9 to Groups-G1, G2 and G5.

The ear thickness was measured daily using digital caliper. On Day 10,animal's blood was withdrawn by retro-orbital plexus. Blood serum wasseparated for the estimation of nitric oxide level by Griess method.Further, all the animals were humanely sacrificed and ear punch biopsieswere collected, weighed and subjected for histopathological analysis,immunohistochemical Ki-67 staining and Myeloperoxidase (MPO) activity.

TABLE 2 Allocation of Animals No. of Groups Treatment Volume & DoseRoute Animals G1 TPA + 3HX 20 μL + 20 μL of 1:4 Topical 6 G2 TPA + 3HX20 μL + 20 μL of 1:8 Topical 6 G3 TPA + 3HX 20 μL + 500 mg/kg Oral 6 G4TPA + 3HX 20 μL + 1000 mg/kg Oral 6 G5 TPA + D&E 20 μL + 20 μL of stockTopical 6 (1 ug D + 100 μg E) G6 TPA + D&E 20 μL + 1 mg/kg D Oral 6 and100 mg/kg E G7 TPA Control 20 μL TPA + 0.25% Oral 6 Na-CMC

Observations/Calculations Body Weight

Body weight of all the animals was recorded from day 0 to day 10. The %change in body weight for each animal was calculated using the givenformula:

% Body Weight Change=Body Weight at ‘X’ day-Body weight at day ‘0’×100Body weight at day ‘0’

“x”: Day 1, 2, 3, 4, 5, 6, 7, 8, 9, 10

Ear Thickness Measurement

Upper, middle and lower ear thickness of both right and left ear ofGroup G1 and right ear of Group G2 to G7 was measured and recorded dailyfrom Day 0 to Day 9 using digital caliper (MITUTOYO) and their averagevalue was considered for further calculations.

Mean absolute ear thickness (in mm) are shown in FIGS. 24-25.

Ear Thickness Change (ETC)

Mean ear thickness change or ear edema was calculated based on theabsolute ear thickness values.

Ear thickness change (mm) (x)={Absolute ear thickness (mm) at “x” TimePoint}−{Absolute ear thickness (mm) at day 0}

“x”: day 0, 1, 2, 3, 4, 5, 6, 7, 8, 9

Ear thickness change are shown in FIGS. 24-25.

Photograph & Ear Biopsy Weight

Photographs of ear was taken for each animal and presented. Mean earbiopsy weight (4 mm punch biopsy) was calculated and represented intabulated and graphical form.

Percentage Inhibition of Ear Inflammation

Percentage inhibition of ear inflammation was calculated for individualanimal using the formula:

=Control ETC (mm)−Test ETC (mm)/Control ETC (mm)×100

% Inhibition was tabulated and represented in graphical form.

Histopathology and Immunohistochemistry

Hematoxylin & Eosin dye stained ear histopathological photographs weresubjected for epidermal ear thickness measurement using UTHSCSA Imagetool, Version 3.0. The software was calibrated by Motic calibrated slidefor 10× magnification and ear thickness was selected randomly at fourpoints each for both upper and lower epidermis. Data was calculated asmean and SEM for each group and represented in tabulated and graphicalform

Myeloperoxidase (MPO) Activity

MPO activity was performed by colorimetric method as described by Rajpet al. (2007). Briefly, three punch biopsy ear (4 mm) tissue samples ineach group were pooled, weighed and homogenized (100 mg/3 mL) in 50 mM/Lphosphate buffer containing 0.5% CTAB. Homogenized samples weresonicated for 10 s, freezed and thawed at 20-30° C. for three times, andcentrifuged at 12000 rpm and 4° C. for 25 min. 250 μL of supernatant wasbe mixed with 625 μL of phosphate buffer (50 mmol/l, pH 6) containing0.167 mg/ml o-dianisidine dihydrochloride and 125 μL hydrogen peroxide(0.0005%). MPO activity was calculated by using the formulae andrepresented in graphical and tabular form.

IU/mL=ΔA×final volume in cuvette/8.3×volume of sample added(μL)×dilution factor.

Where ΔA is the average of change in absorbance per minute.

IU/gm tissue=(IU/mL)/(gm tissue/mL)

Serum Nitric Oxide Level

Nitric oxide levels was also measured by colorimetric assay using Griessreagent. Briefly, equal volume of serum was mixed with Griess reagentand incubated for 15 min at 37° C.

Absorbance was measured at 546 nm and percentage inhibition of nitricoxide level was calculated using the formula:

=Control Abs−Test Abs/Control Abs×100

% Inhibition was tabulated and represented in graphical form.

Statistical Analysis

All data were expressed in Mean±SEM and suitably analyzed by Two wayANOVA and One-way ANOVA followed by Posthoc Bonferroni and Dunett's testrespectively for statistical significance. *P<0.05, **P<0.01 and***P<0.001 for TPA control Vs vehicle or treated group.

Study Deviation

The method adopted for MPO estimation was changed as stated in thesection on MYELOPEROXIDASE (MPO) ACTIVITY. Statistical analysis was doneby Two way and One way ANOVA followed by Posthoc-Bonferroni and Dunnet'stest respectively, as it was appropriate for study data analysis.

Results Effect of Treatments on Body Weight Change

In the present study, the body weight was recorded daily for 10consecutive days (i.e day 0 to day 9) in all the experimental groups. Nosignificant changes in body weight were observed in any of theexperimental group (Table 3; FIG. 3). % body weight change was alsocalculated for all the experimental groups. Marginal reduction in % bodyweight change was observed on oral administration of 3-HX at high doseof 1000 mg/kg and D & E treated animals (Table 4; FIG. 4).

TABLE 3 Effect of 3-HX and D & E treatment on Body Weight (Unit: gm;Mean ± SEM) Treatment Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7Day 8 Day 9 Day 10 3-HX (1:4); 27.4 ± 2.7 28.5 ± 2.4 28.2 ± 2.4 27.8 ±2.4 27.9 ± 2.3 27.8 ± 2.2 27.8 ± 2.3 28.2 ± 2.3 28.3 ± 2.3 28.3 ± 2.327.7 ± 2.4 Topical 3-HX (1:8); 27.5 ± 2.0 28.4 ± 1.6 28.0 ± 1.6 28.0 ±1.6 27.9 ± 1.6 27.8 ± 1.5 28.3 ± 1.6 28.4 ± 1.6 28.9 ± 1.6 28.1 ± 1.428.9 ± 1.6 Topical 3-HX 27.6 ± 1.6 27.7 ± 1.6 27.3 ± 1.6 27.4 ± 1.6 27.5± 1.6 27.2 ± 1.6 27.3 ± 1.6 27.6 ± 1.6 28.0 ± 1.5 28.1 ± 1.5 28.1 ± 1.4(500 mg/kg); Oral 3-HX 27.5 ± 1.7 27.97 ± 1.6  27.6 ± 1.5 27.8 ± 1.627.9 ± 1.5 27.8 ± 1.5 27.2 ± 1.8 27.0 ± 1.7 26.4 ± 2.0 26.1 ± 2.2 26.6 ±2.2 (1000 mg/kg); Oral D and E; 27.7 ± 1.6 28.1 ± 1.4 28.2 ± 1.3 28.3 ±1.3 28.3 ± 1.3 28.3 ± 1.2 28.6 ± 1.2 28.8 ± 1.2 28.9 ± 1.2 29.2 ± 1.229.7 ± 1.2 Topical D and E; 28.0 ± 1.5 26.1 ± 1.3 25.64 ± 1.3  25.9 ±1.2 26.7 ± 1.2 26.9 ± 1.3 25.9 ± 1.4 25.5 ± 1.5 25.1 ± 1.6 24.8 ± 1.624.3 ± 1.7 Oral Na-CMC 28.1 ± 1.4 27.5 ± 1.2 27.4 ± 1.1 27.8 ± 1.2 27.5± 1.1 27.6 ± 1.0 27.5 ± 0.9 28.3 ± 0.8 28.7 ± 0.8 28.4 ± 0.8 28.8 ± 0.9

Each column represents mean±SEM of n=6 mice. Data was statisticallyanalyzed by Two way ANOVA followed by Posthoc Bonferrini's test. Nostatistical significance was obtained in any of the experimental group.

FIG. 3 illustrates effects of 3-HX and D & E treatment on Body weightchange.

TABLE 4 Effect of 3-HX and D & E treatment on % Body Weight ChangeTreatment Day-1 Day-2 Day-3 Day-4 Day-5 Day-6 Day-7 Day-8 Day-9 Day-103-HX (1:4); 4.6 3.6 2.3 2.9 2.6 2.6 3.9 4.2 4.1 2.4 Topical 3-HX (1:8);4.1 2.4 2.4 2.1 1.9 3.8 3.9 5.9 3.2 6.1 Topical 3-HX 0.5 0.5 −0.8 −0.3−1.3 −1 0 1.7 2.1 2.3 (500 mg/kg); Oral 3-HX 2 0.8 1.4 1.9 1.5 −1.3 −1.6−4.6 −5.7 −3.9 (1000 mg/kg); Oral D and E; 1.7 1.9 2.5 2.6 2.7 3.6 4.65.1 6 7.9 Topical D and E; −6.9 −8.4 −7.4 −4.4 −3.6 −7.3 −8.5 −10 −11.3−13.2 Oral Vehicle −1.8 −2.2 −0.8 −2 −1.6 −1.6 1.2 2.6 1.5 3

Each column represents mean±SEM of n=6 mice.

FIG. 4 illustrates effects of 3-HX and D & E treatment on % Body WeightChange.

Effect of Treatments on Absolute Ear Thickness

In the present study, the absolute ear thickness was calculated dailyfor 10 consecutive days (i.e day 0 to day 9) in all the experimentalgroups (Table 5). Significant increase (P<0.001) in ear thickness wasobserved in the TPA control (Group 7/Right ear) when compared with thevehicle control (Group 7/Left ear) in entire treatment schedule.

Topical application of 3-HX at both the tested ratios of 1:4 and 1:8significantly reduced the absolute ear thickness when compared with TPAcontrol. Similarly, topical application of D & E also significantlyreduced the absolute ear thickness when compared with TPA control(P<0.001) (FIG. 5).

Oral administration of 3-HX at dose of 500 mg/kg and 1000 mg/kg wasfound to significantly reduce TPA induced absolute ear thickness. D & Etreatment also markedly reduced absolute ear thickness on oraladministration for 10 days (P<0.001) (FIG. 6).

TABLE 5 Effect of 3-HX and D & E treatment on Ear Thickness (Unit: mm;Mean ± SEM) Treatment Day-0 Day-1 Day-2 Day-3 Day-4 Day-5 3HX, 1:4  0.3± 0.01 0.54 ± 0.04*** 0.54 ± 0.04*** 0.72 ± 0.02*** 0.78 ± 0.05*** 0.68± 0.04*** Topical 3HX, 1:8 0.31 ± 0 0.59 ± 0.04*** 0.54 ± 0.03***  0.7 ±0.01*** 0.87 ± 0.03** 0.67 ± 0.02*** Topical 3HX 0.31 ± 0 0.56 ± 0.05***0.55 ± 0.04*** 0.67 ± 0.04*** 0.81 ± 0.04*** 0.72 ± 0.03*** (500 mg/kg);Oral 3HX 0.31 ± 0 0.64 ± 0.03 0.61 ± 0.02*** 0.75 ± 0.04*** 0.92 ± 0.030.73 ± 0.02*** (1000 mg/kg); Oral D&E, 0.31 ± 0 0.56 ± 0.03*** 0.64 ±0.05*** 0.66 ± 0.04*** 0.82 ± 0.01***  0.8 ± 0.03*** Topical D&E, Oral 0.3 ± 0 0.66 ± 0.03 0.64 ± 0.04*** 0.68 ± 0.05***  0.8 ± 0.05*** 0.79 ±0.05*** TPA  0.3 ± 0  0.7 ± 0.03*** 0.88 ± 0.01*** 0.91 ± 0.02*** 0.95 ±0.04***  0.9 ± 0.02*** Control Vehicle  0.3 ± 0 0.31 ± 0 0.32 ± 0 0.32 ±0 0.33 ± 0 0.34 ± 0.01 Control Treatment Day-6 Day-7 Day-8 Day-9 3HX,1:4 0.75 ± 0.06** 0.68 ± 0.05*** 0.58 ± 0.03*** 0.57 ± 0.04*** Topical3HX, 1:8 0.73 ± 0.05*** 0.67 ± 0.07*** 0.63 ± 0.04*** 0.52 ± 0.02***Topical 3HX 0.77 ± 0.05  0.7 ± 0.04*** 0.68 ± 0.03 0.67 ± 0.03 (500mg/kg); Oral 3HX 0.74 ± 0.03** 0.68 ± 0.02*** 0.71 ± 0.02  0.8 ± 0.04**(1000 mg/kg); Oral D&E, 0.67 ± 0.03*** 0.51 ± 0.02*** 0.52 ± 0.02***0.49 ± 0.02*** Topical D&E, Oral 0.85 ± 0.06 0.72 ± 0.08** 0.82 ± 0.16**0.69 ± 0.13 TPA 0.83 ± 0.02***  0.8 ± 0.03*** 0.73 ± 0.03*** 0.72 ±0.05*** Control Vehicle 0.34 ± 0.01 0.35 ± 0.01 0.33 ± 0.1 0.33 ± 0.1Control Each column represents mean ± SEM of n = 6 mice. Data wasstatistically analyzed by Two way ANOVA followed by Posthoc Bonferronitest. ***P < 0.001, **P < 0.01 and *P < 0.05 for vehicle control Vs TPAcontrol; Treatment Vs TPA control.

FIG. 5 illustrates effects of topical application of 3-HX and D & E onAbsolute Ear Thickness in TPA induced mouse.

FIG. 6 illustrates effects of oral application of 3-HX and D & E onAbsolute Ear Thickness in TPA induced mouse.

Effect of Treatment on Ear Thickness Change

The change in ear thickness was also calculated from the absolute earthickness for all the experimental groups to find the edema caused byinflammation. In the TPA control group significant elevation in earthickness was observed when compared with vehicle (Solvent) control(P<0.001) (Table 6).

Topical application of 3-HX at the tested ratios significantly decreasedthe ear edema up to Day 5 in comparison with TPA control (P<0.001).However, from Day 6 onwards marginal decrease in ear thickness changewas observed. Similarly, significant decrease in ear thickness changewas also observed on D & E topical application for entire treatmentschedule except for Day 5 and Day 6 (P<0.001) (FIG. 7).

Oral application of 3-HX at the two tested concentration significantlydecreased the ear edema up to Day 5 in comparison with TPA control(P<0.001). However, from Day 6 onwards marginal decrease in earthickness change was observed. Similarly, significant decrease in earthickness change was also observed on oral administration of D & E up toDay 3 only when compared with TPA control (P<0.001) However, marginaldecrease in ear thickness change was observed on further D & E treatment(FIG. 8).

TABLE 6 Effect of 3-HX and D & E treatment on Ear Thickness Change(Unit: mm; Mean ± SEM) Treatment Day-1 Day-2 Day-3 Day-4 Day-5 3HX, 1:40.24 ± 0.01 0.24 ± 0.04*** 0.42 ± 0.04* 0.48 ± 0.02 0.38 ± 0.05 Topical3HX, 1:8 0.28 ± 0.00 0.23 ± 0.04*** 0.39 ± 0.03** 0.56 ± 0.01 0.36 ±0.03** Topical 3HX, 0.26 ± 0.00 0.25 ± 0.05*** 0.37 ± 0.04*** 0.51 ±0.04 0.41 ± 0.04* 500mpk, Oral 3HX, 0.32 ± 0.00  0.3 ± 0.03*** 0.44 ±0.02* 0.6 ± 0.04 0.42 ± 0.03* 1000mpk, Oral D&E, Topical 0.25 ± 0.000.33 ± 0.03*** 0.35 ± 0.05*** 0.51 ± 0.04 0.49 ± 0.01 D&E, Oral 0.35 ±0.00 0.33 ± 0.03*** 0.37 ± 0.04***  0.5 ± 0.05 0.48 ± 0.05 TPA Control0.39 ± 0.00*** 0.58 ± 0.03*** 0.61 ± 0.01*** 0.64 ± 0.02*** 0.59 ±0.04*** Vehicle 0.01 ± 0 0.02 ± 0 0.02 ± 0 0.03 ± 0 0.04 ± 0.00 ControlTreatment Day-6 Day-7 Day-8 Day-9 3HX, 1:4 0.45 ± 0.04 0.42 ± 0.06 0.28± 0.05 0.27 ± 0.03 Topical 3HX, 1:8 0.42 ± 0.02 0.37 ± 0.05 0.33 ± 0.070.22 ± 0.04* Topical 3HX, 0.47 ± 0.03 0.39 ± 0.05 0.38 ± 0.04 0.36 ±0.03 500mpk, Oral 3HX, 0.43 ± 0.02 0.37 ± 0.03  0.4 ± 0.02 0.49 ± 0.021000mpk, Oral D&E, Topical 0.36 ± 0.03*  0.2 ± 0.03*** 0.22 ± 0.02**0.18 ± 0.02*** D&E, Oral 0.55 ± 0.05 0.42 ± 0.06 0.52 ± 0.08 0.39 ± 0.16TPA Control 0.53 ± 0.02***  0.5 ± 0.02*** 0.43 ± 0.03*** 0.42 ± 0.03***Vehicle 0.04 ± 0.01 0.04 ± 0.01 0.03 ± 0.01 0.03 ± 0.01 Control Eachcolumn represents mean ± SEM of n = 6 mice. Data was statisticallyanalyzed by Two way ANOVA followed by Posthoc Bonferroni test. ***P <0.001, **P < 0.01 and *P < 0.05 for vehicle control Vs TPA control; andfor treatment Vs TPA control.

FIG. 7 illustrates effects of oral application of 3-HX and D & E on EarThickness Change (Effect of 3-HX and D & E oral treatment on thicknesschange in TPA induced mouse ear).

FIG. 8 illustrates effects of oral application of 3-HX and D & E on EarThickness Change (Effect of 3-HX and D & E oral treatment on thicknesschange in TPA induced mouse ear).

Effects of Treatments on Ear Punch Biopsy Weight

On Day 10, all the experimental animals were euthanized and a standard 4mm ear punch biopsy was collected and weighed. Significant increase inbiopsy weight was observed in TPA control (P<0.01) when compared withvehicle control as depicted in Table 7. Ear punch biopsy weight of 3-HX(1:4; topical) treated mice was found to be significantly reduced incomparison to TPA control (P<0.05). However, marginal reduction in earpunch biopsy weight was observed on topical treatment of 3-HX at theratio of 1:8. Based upon punch biopsy weight, the % activity of 3-HX atboth the tested ratios of 1:4 and 1:8 were calculated and was found tobe 29.34% and 17.58% respectively. Significant reduction in punch biopsyweight was also observed in D & E topical treatment group (P<0.05) whencompared with TPA control. Topical treatment with D & E exhibited 31.40%activity against TPA induced inflammation.

A dose-dependent reduction in ear biopsy weight was observed in 3-HXoral administration when compared with TPA control (FIG. 9). 3-HX atconcentration of 500 mg/kg and 1000 mg/kg exhibited 11.40% and 14.90%activity respectively; however no statistical difference was obtainedamong the 3-HX oral treated and TPA control group. D & E oraladministration also reduced ear punch biopsy thickness when comparedwith TPA control and exhibited 21.93% activity. However, no statisticaldifference was obtained among the D & E treated animals and TPA controlgroup.

TABLE 7 Effect of 3-HX and D & E treatment on Ear Punch Biopsy Weight(Unit: mg; Mean ± SEM) Biopsy Weight Treatment (mg) 3-HX (1:4); Topical7.25 ± 0.33* 3-HX (1:8); Topical 8.46 ± 1.05 3HX-500 mg/kg; Oral 9.09 ±1.14 3HX-1000 mg/kg; Oral 8.73 ± 0.34 D & E; Topical 7.04 ± 0.29* D & E;Oral 8.01 ± 0.58 TPA control 10.26 ± 0.82** Vehicle control 3.08 ± 0.21Each point represents the mean ± SEM of n = 6 mice per group. **P < 0.01for Vehicle Control Vs TPA control and *P < 0.05 for treatment Vs TPAcontrol.

FIG. 9 illustrates effects of 3-HX and D & E treatment on Ear PunchBiopsy Weight (mg).

Photographs of Mice Ears

Photographs of TPA induced inflammation in mice ear were represented forall the experimental groups which demonstrated the gross effect of testitems.

FIGS. 10A-10G illustrates Photographs of ear, including:

Group 1: 3-HX (1:4); Topical: G1A1-G1A6

Group 2: 3-HX (1:8); Topical: G2A1-G2A6

Group 3: 3-HX (500 mg/kg); Oral: G3A1-G3A6

Group 4: 3-HX (1000 mg/kg); Oral: G4A1-G4A6

Group 5: D & E; Topical: G5A1-G5A6

Group 6: D & E; Oral: G6A1-G6A6

Group 7: TPA Control (Right Ear) & Vehicle Control (Left Ear): G7A1-G7A6

Note: G denotes Group Number and A denotes Animal number

Percentage Inhibition

In order to calculate percentage inhibition of ear inflammation on testitem treatment, the basal ear thickness change represented by vehiclecontrol was subtracted from each group. The percentage inhibition of earinflammation by test items was calculated as tabulated in table 8.

FIG. 11 depicts the % inhibition of ear inflammation on topicalapplication of 3-HX and D & E. 3-HX at the ratio of 1:4 exerted 35.96%activity on Day 8 which sustained up to Day 9. 3-HX at the ratio of 1:8exerted maximum inhibition activity of 52.44% on Day 9 treatment.Similarly, topical application of D & E exerted 64.94% inhibitoryactivity from Day 7 which sustained up to Day 9 treatment.

FIG. 12 depicted the % inhibition of ear inflammation on oraladministration of 3-HX and D & E. 3-HX at both the doses of 500 mg/kgand 1000 mg/kg exhibited approximately 32% inhibitory activity on Day 5which subsequently got decreased on further treatment up to Day 9.Similarly, D & E oral administration exerted marginal inhibitoryactivity against TPA induced inflammation.

TABLE 8 % Inhibitory Activity of Ear Inflammation Treatment Day-1 Day-2Day-3 Day-4 Day-5 Day-6 Day-7 Day-8 Day-9 3HX (1:4); 39.86 61.42 32.2527.16 38.41 16.90 17.34 35.96 37.68 Topical 3HX (1:8); 28.99 62.02 37.0113.44 41.63 21.72 28.54 24.72 52.44 Topical 3HX (500 mg/kg); 35.36 59.5540.91 22.25 32.46 12.41 22.76 12.92 15.19 Oral 3HX 17.68 50.94 28.356.45 32.16 21.03 28.17 6.60 −17.05 (1000 mg/kg); Oral D&E; Topical 36.8144.91 43.10 21.44 18.75 34.02 64.94 52.81 60.74 D&E; Oral 9.71 44.2139.96 23.43 19.76 −3.91 17.47 −24.02 8.74

FIG. 11 shows % Inhibition of Ear Inflammation on topical treatment (%Inhibitory activity of 3-HX and D & E on topical application).

FIG. 12 shows % Inhibition of Ear Inflammation on oral treatment (%Inhibitory activity of 3-HX and D & E on oral application).

Histopathological and Immunohistochemistry Findings

The H&E-stained ear sections of all the experimental groups arerepresented in FIG. 13. Edema and inflammatory cell infiltration arescored as nil (−), mild (+), moderate (++) and severe (+++). Moreover,the epidermal thickness was also calculated for all the experimentalgroups. Repeated TPA application resulted in a marked increase in earthickness with almost nil hyperkeratosis and moderate edema. Moreover,severe inflammatory cell infiltration in the dermis was also observed. Asignificant increase in epidermal ear thickness (P<0.01) was alsoobserved in TPA control group when compared with vehicle control (Table9).

Topical administration of 3-HX at the ratio of 1:4 showed almost nilhyperkeratosis and moderate edema. However, severe inflammatory cellinfiltration was observed upon 10 days topical application. Similarly,topical administration of 3-HX at the ratio of 1:8 showed mildhyperkeratosis and edema with severe inflammatory cell infiltration. D &E topical application for 10 consecutive days resulted into moderatehyperkeratosis with moderate edema and infiltration of inflammatorycells. The epidermal thickness was also observed to be significantlyreduced on 3-HX topical treatment at both the ratios when compared withTPA control (P<0.05). However, on D & E topical application nosignificant results were obtained when compared with TPA control (FIG.14).

Oral administration of 3-HX at the low dose of 500 mg/kg resulted intomild hyperkeratosis and edema with moderate to severe infiltration ofinflammatory cells. 3-HX at the high dose of 1000 mg/kg resulted intomild to moderate hyperkeratosis and edema with severe infiltration ofinflammatory cells. Similarly, oral administration of D & E leads tomild hyperkeratosis with moderate edema and severe inflammatory cellinfiltration. The epidermal ear thickness was also found to besignificantly reduced on 3-HX treatment at higher dose of 1000 mg/kgalone (P<0.05) (FIG. 15). However, in other oral treatment groups nosignificant differences were obtained in comparison with TPA control.

FIGS. 30A-30H: Histopathological Findings

Group 1: 3-HX (1:4); Topical. Photographs of H&E-stained mouse earcross-sections of 3-HX (1:4; topical) treated mice in the TPAinflammation model. Reduction in hyperkeratosis and edema was observed(10× magnification).

Group 2: 3-HX (1:8); Topical. Photographs of H&E-stained mouse earcross-sections of 3-HX (1:8; topical) treated mice in the TPAinflammation model. Reduction in hyperkeratosis and edema was observed(10× magnification).

Group 3: 3-HX (500 mg/kg); Oral. Photographs of H&E-stained mouse earcross-sections of 3-HX (500 mg/kg; oral) treated mice in the TPAinflammation model. Reduction in hyperkeratosis and edema was observed(10× magnification).

Group 4: 3-HX (1000 mg/kg); Oral. Photographs of H&E-stained mouse earcross-sections of 3-HX (1000 mg/kg; oral) treated mice in the TPAinflammation model. Reduction in hyperkeratosis and edema was observed(10× magnification).

Group 5: D & E; Topical. Photographs of H&E-stained mouse earcross-sections of D & E topically treated mice in the TPA inflammationmodel. Reduction in hyperkeratosis, edema and inflammatory cellinfiltration was observed (10× magnification).

Group 6: D & E; Oral. Photographs of H&E-stained mouse earcross-sections of D & E orally treated mice in the TPA inflammationmodel. Reduction in hyperkeratosis and edema was observed (10×magnification).

Group 7: TPA Control (Right Ear). Photographs of H&E-stained mouse earcross-sections in the TPA induced inflammation model. Severeinfiltration of inflammatory cells with moderate edema in ears wasobserved (10× magnification).

Group 7: Vehicle control (Left Ear). Photographs of H&E-stained mouseear cross-sections of vehicle control group (10× magnification).

TABLE 9 Effect of Test items Treatment on Epidermal ear thickness (Unit:μm; Mean ± SEM) Treatment Epidermal Ear Thickness Vehicle Control 12.15± 3.08 TPA Control 54.42 ± 7.76** 3-HX (1:4); Topical 36.41 ± 5.16* 3-HX(1:8); Topical 34.06 ± 2.46* 3HX-500 mg/kg; Oral 43.26 ± 3.36 3HX-1000mg/kg; Oral 34.49 ± 3.81* D & E; Topical 37.88 ± 4.85 D & E; Oral 37.48± 3.11 **P < 0.01 for vehicle control compared to TPA Control; *P < 0.05for TPA Control compared to 3-HX treatment group.

FIG. 14 illustrates effects of topical application of 3-HX and D & E onepidermal ear thickness.

FIG. 15 illustrates effects of oral treatment of 3-HX and D&E onepidermal ear thickness.

Myeloperoxidase (MPO) Activity

Table 10 illustrated the effect of test items over enzymemyeloperoxidase, released by neutrophils and macrophages duringinflammation process. In the present study, the enzyme activity wasfound to be markedly enhanced in TPA control group when compared withvehicle control. Topical application of 3-HX at both the ratiosprofoundly inhibited the MPO activity on 10 days of consecutiveapplication in comparison with TPA control. Topical application ofstandard D & E showed maximum inhibition of MPO activity among all thetopical treatment groups (FIG. 16).

Oral administration of 3-HX showed dose-dependent inhibition of enzymeMPO against TPA induced mice ear inflammation. D & E oral treatment alsoobserved to inhibit the MPO levels when compared with TPA control (FIG.17).

TABLE 10 Effect of Test items Treatment on MPO activity in TPA inducedmice ear (Unit: μm; Mean ± SEM) Treatment IU/gm tissue (Mean ± SEM)Vehicle Control 0.094 ± 0.052 TPA Control 0.487 ± 0.184 3-HX (1:4);Topical 0.238 ± 0.044 3-HX (1:8); Topical 0.244 ± 0.037 3HX-500 mg/kg;Oral 0.380 ± 0.051 3HX-1000 mg/kg; Oral 0.339 ± 0.038 D & E; Topical0.196 ± 0.046 D & E; Oral 0.258 ± 0.068

FIG. 16 illustrates effects of topical application of 3-HX and D & E onMPO activity in TPA induced mice ear.

FIG. 17 illustrates effects of oral administration of 3-HX and D&E onMPO activity in TPA induced mice ear.

Nitric Oxide (NO) Activity

Table 11 illustrated the % inhibition of nitric oxide levels in serum ofTPA induced inflammation on treatment of test items. Topical applicationof 3-HX at both the ratios exerted poor inhibitory effect over nitricoxide levels on 10 days of consecutive application. Similarly, topicalapplication of standard D & E also exhibited poor inhibitory responseover serum NO levels.

Oral administration of 3-HX at the dose of 500 mg/kg and 1000 mg/kgexhibited 32.53% and 30.62% inhibitory activity respectively over serumnitric oxide content against TPA induced inflammation. D & E oraltreatment also observed to exert 25.10% inhibitory activity (FIG. 18).

TABLE 11 Effect of Test items Treatment on % Inhibition of Nitric oxidelevel in serum of TPA induced inflammation Treatment % Inhibition 3-HX(1:4); Topical (n = 6) 5.35 3-HX (1:8); Topical (n = 3) 7.10 3-HX (500mg); Oral (n = 4) 32.53 3-HX (1000 mg); Oral (n = 4) 30.62 D & E;Topical (n = 4) 6.02 D & E; Oral (n = 4) 25.10 Note: n represents plasmasamples size analyzed for NO activity

FIG. 18 shows % Inhibition of serum Nitric oxide content on 3-HX and D&Etreatment (Effect of 3-HX and D&E on serum nitric oxide level).

These examples demonstrate anti-psoriatic potential of 3-HX and D & E onboth topical and oral treatment in TPA induced ear inflammation inC57BL/6 mice. The results demonstrated no significant changes in bodyweight among all the experimental groups. However, in % body weightchange, a marginal reduction was observed on oral administration of D &E and high dose of 3-HX, although both ranged within the tolerablerange.

In the present study results marked induction of ear inflammation onrepeated TPA application was observed as indicated by significantincrease in ear thickness. Topical application of TPA is reported toinduce cutaneous inflammation and epidermal hyperplasia (Clark et al.,1985). The TPA application resulted in a series of events of numerouscellular, biochemical, and molecular changes that eventually lead to thepathological alterations of the mouse skin (Kensler et al., 1987;Nakamura et al., 1998, 2000). Among the topically treated groups, highconcentration of 3-HX (1:4) and D&E was found to exert maximumanti-inflammatory activity as indicated by significant reduction in earthickness. Among the orally administered experimental groups, low doseof 3-HX (500 mg/kg) exhibited maximum reduction in ear thicknessfollowed by D & E and high dose of 3-HX (1000 mg/kg).

The effect of topical and oral treatment of test items was also examinedover TPA-induced ear thickness change which also represents inflammatoryedema. Topical application of 3-HX (1:4) exerted maximum reduction inear thickness change followed by D & E and low concentration of 3-HX(1:8). Among the orally administered groups, low dose of 3-HX (500mg/kg) rendered maximum reduction in ear thickness change followed by D& E and high dose of 3-HX (1000 mg/kg). The overall % inhibitoryactivity of test items based upon the ear thickness and ear thicknesschange demonstrated that topical application of D & E resulted inmaximum inhibition of ear thickness followed by 3-HX (1:8) and 3-HX(1:4). On the other side, oral application of all the test items exertedmarginal inhibitory activity against TPA induced ear inflammation inmice.

The effect of test items was further evaluated by measuring the standard4 mm punch biopsy weight after completion of treatment schedule. 10consecutive day TPA application resulted in profound increase in punchbiopsy weight of mice ear suggesting the chronic inflammatory response.Topical application of D & E exerted maximum reduction in ear punchbiopsy weight followed by 3-HX (1:4) and 3-HX (1:8). Oral administrationof 3-HX and D & E resulted in marginal reduction in ear punch biopsyweight suggesting the beneficial effect of test items on topicalapplication in comparison with oral treatment.

To further explore the anti-psoriatic potential of test items, theepidermal ear thickness was evaluated in histopathological photographs.The histopathogical findings were in accordance with punch biopsy weightresults where topical application of test items was found to exertbetter effect against TPA induced increase in epidermal ear thicknessear in comparison with orally treated experimental groups. Moreover,marked reduction in hyperkeratosis, ear edema and inflammatory cellinfiltration was also observed among all the treatment groups whencompared with TPA control.

In the present study, the effect of test items was also investigatedover inflammatory biomarkers viz., enzyme myeloperoxidase and nitricoxide in TPA model of inflammation. In the present study marked increasein MPO activity was observed in TPA control mice ear homogenate which isin accordance with the previous reports (Lee et al. 2009). MPO iscommonly used as an index of granulocyte infiltration and the enzymeinhibition is indicative of anti-inflammatory activity in the chronicinflammation model (Ajuebor et al. 2000). Topical application of D & Eresulted in maximum suppression of enzyme activity followed by 3-HX(1:4) and 3-HX (1:8). Among the orally treated experimental groups, D &E exerted marked suppression of enzyme activity whereas 3-HX exhibitedmarginal activity.

Similarly, marked increase in nitric oxide levels were observed in TPAcontrol group. In the present study, oral administration of test itemspartially inhibited the generation of nitric oxide content against TPAinduced mice inflammation.

The embodiments have been described as demonstrated anti-psoriaticpotential of 3-HX and D & E on both topical and oral application inTPA-induced ear inflammation model using C57BL/6 mice. Based upon thepresent findings, it is suggested that topical application of D & E and3-HX may act as potential therapeutic intervention for the treatment ofinflammatory skin diseases like eczema through inhibition ofmyeloperoxidase activity. Future investigations are recommended in-orderto find out the mechanism of action which is required for thedevelopment of novel therapeutics for the treatment of eczema.

The following are incorporated by reference:

-   Rajp A, Adu D and Savage C O (2007). Meta-analysis of    myeloperoxidase G-463/A polymorphism in anti-neutrophil cytoplasmic    autoantibody-positive vasculitis. Clinical and Experimental    Immunology. 149: 251-256.-   Clark S D, Wilhelm S M, Stricklin G P, Welgus H G (1985)    Coregulation of collagenase and collagenase inhibitor production by    phorbol myristate acetatein human skin fibroblasts. Arch. Biochem.    Biophys. 241, 36-44.-   Kensler T W, Egner P A, Moore K G, Taffe B G, Twerdok L E, Trush M    A (1987) Role of inflammatory cells in the metabolic activation of    polycyclic aromatic hydrocarbons in mouse skin. Toxicol. Appl.    Pharmacol. 90, 337-346.-   Nakamura Y, Murakami A, Ohto Y, Torikai K, Tanaka T, Ohigashi    H (1998) Suppression of tumor promoter induced oxidative stress and    inflammatory responses in mouse skin by superoxide generation    inhibitor 10-acetoxychavicol acetate. Cancer Res. 58, 4832-4839.-   Nakamura Y, Torikai K T, Ohto Y, Murakami A, Tanaka T, Ohigashi    H (2000) A simple phenolic antioxidant protocatechuic acid enhances    tumor promotion and oxidative stress in female ICR mouse skin: dose-    and timing-dependent enhancement and involvement of bioactivation by    tyrosinase. Carcinogenesis 21, 1899-1907.-   Lee D Y, Choi G, Yoon T, Cheon M S, Choo B K, Kim H K (2009)    Anti-inflammatory activity of Chrysanthemum indicum extract in acute    and chronic cutaneous inflammation. Journal of Ethnopharmacology    123:149-154.-   Ajuebor M N, Singh A, Wallace J L (2000) Cyclooxygenase-2-derived    prostaglandin D(2) is an early anti-inflammatory signal in    experimental colitis. American Journal of Physiology    Gastrointestinal and Liver Physiology 279, G238-G244.

LIST OF TABLES Table No. Heading/Title  1. Oral purgative ED₅₀ values ofthe anthraquinones.  2. Allocation of animals  3. Effect of 3-HX and D &E treatment on Body Weight  4. Effect of 3-HX and D & E treatment on %Body Weight Change  5. Effect of 3-HX and D & E treatment on EarThickness  6. Effect of 3-HX and D & E treatment on Ear Thickness Change 7. Effect of 3-HX and D & E treatment on Ear Punch Biopsy Weight  8. %Inhibitory Activity of Ear Inflammation  9. Effect of Test itemsTreatment on Epidermal ear thickness 10. Effect of Test items Treatmenton MPO activity in TPA induced mice ear 11. Effect of Test itemsTreatment on % Inhibition of Nitric oxide level in serum of TPA inducedinflammation

ABBREVIATIONS USED

Abbreviation Explanation ° C. Degree Centigrade % Percentage μlMicroliter μm Micrometer ANOVA Analysis of variance CPCSEA Committee forthe Purpose of Control and Supervision of Experiments on Animals DMSODimethylsulfoxide DRF Dabur Research Foundation ETC Ear Thickness ChangeM Molar mg milligram ml milliliter ml/kg milliliter/kilogram mmmillimeter mM millimolar SEM Standard Error Mean SD Standard DeviationTPA 12-O-Tetradecanoylphorbol-13 -Acetate IHC Immunohistochemistry IUInternational Unit

FIG. 20 illustrates the effect on live cells of three combinations ofherbs each in three concentrations 1:40, 1:20 and 1:10. C1 includesSheng Di Huang, C2 includes Jin Yin Hua, Da Huang, Mu Dan Pi and Di GuPi, and C3 includes Xian He Cao and Chun Gen Pi. FIG. 20 illustratesthat each of these three example combinations provides a reduction ofthe live cell numbers particularly in higher concentrations, while C1and C2 appear to be more effective than C3. Each of C1 and C2 includesone or two of the three herbs Sheng Di Huang, Jin Yin Hua and Da Huang,while C3 does not. In addition, the combination of four herbs in C2appears to be more effective than the single herb in C1.

FIGS. 21-23 illustrate plots for each of the eighteen herbs of growth asa percentage of control versus dilution factor. FIGS. 21-23 illustratethat the three herbs Sheng Di Huang, Da Huang and Jin Yin Hua are mosteffective, while the four herbs Mu Dan Pi, Di Gu Pi, Xian He Cao andChun Gen Pi are effective, and the eleven herbs Zi Cao, Xuan Shen, ShiGao, Bai Shao, Chi Shao, Hong Hua, Da Qing Ye, Qing Dai, Bai Zhu, ShiWei and Rou Gui are somewhat less effective.

FIG. 24 illustrates the effect on live cells of five combinations ofherbs each in two concentrations 1:40 and 1:80. Cal includes acombination of all eighteen herbs, C2 includes a combination of Jin YinHua, Da Huang, Mu Dan Pi and Di Gu Pi, C3 includes a combination of DaHuang, Sheng Di Huang and Jin Yin Hua, C4 and C5 include combinations ofthe other eleven herbs (Zi Cao, Xuan Shen, Shi Gao, Bai Shao, Chi Shao,Hong Hua, Da Qing Ye, Qing Dai, Bai Zhu, Shi Wei and Rou Gui) of theeighteen herbs, i.e., not in combination with any of the seven herbs(Sheng Di Huang, Da Huang, Jin Yin Hua, Mu Dan Pi, Di Gu Pi, Xian He Caoand Chun Gen Pi). Specifically, C4 includes Shi Gao, Shi Wei, Bai Zhu,Rou Gui, Hong Hua and C5 includes Zi Cao, Xuan Shen, Chi Shao, Bai Shao,Da Qin Ye, and Qing Dai. FIG. 24 illustrates that combinations of alleighteen herbs, as well as combinations of the three herbs (Sheng DiHuang, Da Huang and Jin Yin Hua) are most effective, while thecombination C2 was less effective, and the combinations C4 and C5 werestill less effective.

It is contemplated, as people with ordinary skill in the art would do,that the newly separated compounds may be each individually or incombination used as an ingredient to prepare a pharmaceuticalcomposition for a particular treatment purpose. As it is the status ofthe art in the pharmaceutical industry, once substantially purepreparations of a compound are obtained, various pharmaceuticalcompositions or formulations can be prepared from the substantially purecompound using conventional processes or future developed processes inthe industry. Specific processes of making pharmaceutical formulationsand dosage forms (including, but not limited to, tablet, capsule,injection, syrup) from chemical compounds are not part of the inventionand people of ordinary skill in the art of the pharmaceutical industryare capable of applying one or more processes established in theindustry to the practice of the present invention. Alternatively, peopleof ordinary skill in the art may modify the existing conventionalprocesses to better suit the compounds of the present invention. Forexample, the patent or patent application databases provided at USPTOofficial website contain rich resources concerning making pharmaceuticalformulations and products from effective chemical compounds. Anotheruseful source of information is Handbook of Pharmaceutical ManufacturingFormulations, edited by Sarfaraz K. Niazi and sold by Culinary &Hospitality Industry Publications Services, which is incorporated byreference.

While the invention has been described in terms of several embodiments,those skilled in the art will recognize that the invention is notlimited to the embodiments described, but can be practiced withmodification and alteration within the spirit and scope of the appendedclaims. The description is thus to be regarded as illustrative insteadof limiting of the invention as set forth in the appended claimsincluding structural and functional equivalents thereof.

1-49. (canceled)
 50. A method of treating eczema, comprising: measuringan elevated level or combination of levels of one or more of TNF-α,TARC, VEGF, IL-6, IgE, JAK-1 or JAK-3 in a first bodily sample extractedfrom a patient; formulating an eczema diagnosis for the patient based onsaid measuring said elevated level or combination of levels; applying amedicinal composition that comprises 1.0 wt. %-15.0 wt. % of an herbalcombination of Da Huang, Sheng Di Huang and Jin Yin Hua to said affectedskin area to treat said eczema.
 51. The method of claim 50, furthercomprising: measuring a reduced level or combination of levels of saidone or more of TNF-α, TARC, VEGF, IL-6, IgE, JAK-1 or JAK-3, in a secondbodily sample extracted from the patient after applying said medicinalcomposition; and repeating the applying of said medicinal composition tosaid affected skin area based on said measuring said reduced level orcombination of levels.
 52. The method of claim 50, wherein the medicinalcomposition comprises 2.5 wt. % or more of said herbal combination of DaHuang, Sheng Di Huang and Jin Yin Hua.
 53. The method of claim 51,wherein the medicinal composition comprises 10 wt. % or less of saidherbal combination of Da Huang, Sheng di Huang and Jin Yin Hua.
 54. Themethod of claim 50, wherein the medicinal composition comprises 2.5 wt.%-5.0 wt. % of said herbal combination of Da Huang, Sheng Di Huang andJin Yin Hua.
 55. The method of claim 50, wherein the medicinalcomposition comprises an effective dose between 20-160 mg/kg.
 56. Themethod of claim 50, wherein the medicinal composition comprises aneffective dose between 1-6 grams of said herbal combination for a 50 kgpatient or 2-12 grams for a 100 kg patient.
 57. The method of claim 56,wherein said effective dose comprises 0.2-1.2 grams of Da Huang, 0.6-3.6grams of Sheng Di Huang and 0.2-1.2 grams of Jin Yin Hua for a 50 kgpatient or 0.4-2.4 grams of Da Huang, 1.2-7.2 grams of Sheng Di Huangand 0.4-2.4 grams of Jin Yin Hua for a 100 kg patient.
 58. The method ofclaim 50, wherein the medicinal composition is formulated as a shampooand applied to the scalp.
 59. The method of claim 50, wherein themedicinal composition is formulated as a shampoo, conditioner, cream,lotion, ointment or other topical scalp or hair treatment.
 60. Themethod of claim 50, wherein the medicinal composition is formulated as acream, lotion, ointment or other topical skin treatment.
 61. A method ofdiagnosing eczema, comprising: measuring elevated levels of two or moreof TNF-α, TARC, VEGF, IL-6, IgE, JAK-1 or JAK-3 in a first bodily sampleextracted from a patient; and formulating an eczema diagnosis for thepatient based on said measuring said elevated levels.
 62. A topicaleczema medicine, comprising a shampoo, conditioner, cream, lotion,ointment or other topical skin, scalp or hair treatment comprising 1.0wt. %-15.0 wt. % of an herbal combination of Da Huang, Sheng Di Huangand Jin Yin Hua.
 63. The medicine of claim 62, comprising 2.5 wt. % ormore of said herbal combination of Da Huang, Sheng Di Huang and Jin YinHua.
 64. The medicine of claim 63, comprising 10 wt. % or less of saidherbal combination of Da Huang, Sheng di Huang and Jin Yin Hua.
 65. Themedicine of claim 62, comprising 2.5 wt. %-5.0 wt. % of said herbalcombination of Da Huang, Sheng Di Huang and Jin Yin Hua.
 66. Themedicine of claim 62, comprising a predetermined number of effectivedoses between 20-160 mg/kg.
 67. The medicine of claim 62, comprising apredetermined number of effective doses between 1-6 grams of said herbalcombination for a 50 kg patient or 2-12 grams for a 100 kg patient. 68.The medicine of claim 67, wherein said effective doses each comprise0.2-1.2 grams of Da Huang, 0.6-3.6 grams of Sheng Di Huang and 0.2-1.2grams of Jin Yin Hua for a 50 kg patient or 0.4-2.4 grams of Da Huang,1.2-7.2 grams of Sheng Di Huang and 0.4-2.4 grams of Jin Yin Hua for a100 kg patient.
 69. A topical eczema medical kit, comprising: a test kitfor measuring a level or combination of levels of one or more of TNF-α,TARC, VEGF, IL-6, IgE, JAK-1 or JAK-3 in a bodily sample, and forindicating a diagnostic result based on said measuring of said level orcombinations of levels in said bodily sample; and a shampoo,conditioner, cream, lotion, ointment or other topical skin, scalp orhair treatment comprising 1.0 wt. %-15.0 wt. % of an herbal combinationof Da Huang, Sheng Di Huang and Jin Yin Hua.
 70. The medical kit ofclaim 69, further comprising a prognostic test kit for measuring a levelor combination of levels of one or more of TNF-α, TARC, VEGF, IL-6, IgE,JAK-1 or JAK-3 in a second bodily sample after a prognostic period oftreatment, and indicating a prognostic result based on said measuring ofsaid level or combination of levels in the second bodily sample.
 71. Themedical kit of claim 69, wherein the shampoo, conditioner, cream,lotion, ointment or other topical skin, scalp or hair treatmentcomprises 2.5 wt. % or more of said herbal combination of Da Huang,Sheng Di Huang and Jin Yin Hua.
 72. The medical kit of claim 71, whereinthe shampoo, conditioner, cream, lotion, ointment or other topical skin,scalp or hair treatment comprises 10 wt. % or less of said herbalcombination of Da Huang, Sheng di Huang and Jin Yin Hua.
 73. The medicalkit of claim 69, wherein the shampoo, conditioner, cream, lotion,ointment or other topical skin, scalp or hair treatment comprises 2.5wt. %-5.0 wt. % of said herbal combination of Da Huang, Sheng Di Huangand Jin Yin Hua.
 74. The medical kit of claim 69, wherein the shampoo,conditioner, cream, lotion, ointment or other topical skin, scalp orhair treatment comprises an effective dose between 20-160 mg/kg.
 75. Themedical kit of claim 69, wherein the shampoo, conditioner, cream,lotion, ointment or other topical skin, scalp or hair treatmentcomprises an effective dose between 1-6 grams of said herbal combinationfor a 50 kg patient or 2-12 grams for a 100 kg patient.
 76. The medicalkit of claim 75, wherein said effective dose comprises 0.2-1.2 grams ofDa Huang, 0.6-3.6 grams of Sheng Di Huang and 0.2-1.2 grams of Jin YinHua for a 50 kg patient or 0.4-2.4 grams of Da Huang, 1.2-7.2 grams ofSheng Di Huang and 0.4-2.4 grams of Jin Yin Hua for a 100 kg patient.